人乳腺癌细胞在体外和体内的抗原脱落

Antigen shedding by human breast-cancer cells in vitro and in vivo.

作者信息

Nordquist R E, Anglin J H, Lerner M P

出版信息

Br J Cancer. 1978 May;37(5):776-9. doi: 10.1038/bjc.1978.115.

DOI:10.1038/bjc.1978.115

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2009636/

Abstract

Human breast cancer cells were adapted to chemically defined medium in order to recover naturally shed glycoproteins. Sephadex G-150 chromatography of these glycoproteins revealed 1 major and 2 minor peaks. When the cells were grown in the presence of 3H-leucine, the radioactive shed proteins had a Sephadex profile identical to the unlabelled shed glycoproteins. PAGE analysis of these proteins showed 5 major bands. Antigenically similar proteins were found in the serum of female nude mice bearing BOT-2 tumours, but not in controls.

摘要

为了回收自然脱落的糖蛋白，将人乳腺癌细胞适应于化学成分明确的培养基。对这些糖蛋白进行葡聚糖G - 150层析，显示出1个主峰和2个次峰。当细胞在3H - 亮氨酸存在的情况下生长时，放射性脱落蛋白的葡聚糖层析图谱与未标记的脱落糖蛋白相同。对这些蛋白进行聚丙烯酰胺凝胶电泳（PAGE）分析，显示出5条主要条带。在携带BOT - 2肿瘤的雌性裸鼠血清中发现了抗原相似的蛋白，但在对照组中未发现。

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