DNA放射性标记对紫外线照射的人成纤维细胞切除修复影响的研究。
An investigation of the effect of radioactive labeling of DNA on excision repair in UV-irradiated human fibroblasts.
作者信息
Ehmann U K, Friedberg E C
出版信息
Biophys J. 1980 Aug;31(2):285-91. doi: 10.1016/S0006-3495(80)85058-2.
Abstract
Previous studies on the kinetics of thymine dimer excision and unscheduled DNA synthesis in UV-irradiated human fibroblasts showed a significant discrepancy in these two parameters (Ehmann et al., 1978. Biophys. J. 22: 249). In the present study we have investigated the effect of the level of the radioactive isotope used for labeling cells on the kinetics of a parameter that directly measures thymine dimer excision. We find no significant differences in the kinetics of this parameter in cells lightly or heavily labeled with radioactive thymidine.
摘要
先前关于紫外线照射的人成纤维细胞中胸腺嘧啶二聚体切除动力学和DNA非预定合成的研究表明，这两个参数存在显著差异（埃曼等人，1978年。《生物物理杂志》22: 249）。在本研究中，我们研究了用于标记细胞的放射性同位素水平对直接测量胸腺嘧啶二聚体切除的一个参数动力学的影响。我们发现，用放射性胸苷轻度或重度标记的细胞中，该参数的动力学没有显著差异。
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