Soule H R, Linder E, Edgington T S
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1332-6. doi: 10.1073/pnas.80.5.1332.
A large number of hybridomas producing antibodies to the membrane fraction of a human mammary carcinoma cell line have been produced. Among those hybridoma antibodies that bound to exposed surface membrane epitopes on the human mammary carcinoma cell line BT-20, one has been found to bind to all tested breast carcinoma cell lines as well as to a number of other human carcinomas by solid-phase radioimmunoassay. This hybridoma antibody, designated 10-3D2, does not bind to normal mammary epithelium, a variety of control cell lines, or selected normal human tissue homogenates. The 10-3D2 hybridoma antibody identifies by immunoprecipitation a membrane 126-kilodalton protein which is indistinguishable in different tumor cells. It is glycosylated by reference to incorporation of [3H]glucosamine and also is phosphorylated by reference to incorporation of 32P. It is present in a finely granular pattern on the cell surface of mammary carcinoma cells with concentration at the sites of apparent cell-cell contact. This 126-kilodalton phosphoglycoprotein, which we have consistently been unable to identify by immunoprecipitation of intrinsically labeled normal cells, may represent a heretofore unrecognized tumor-associated marker for human mammary carcinomas as well as certain other types of human neoplasms.
已经产生了大量针对人乳腺癌细胞系膜组分的杂交瘤。在那些与人乳腺癌细胞系BT - 20上暴露的表面膜表位结合的杂交瘤抗体中，通过固相放射免疫测定发现有一个抗体能与所有测试的乳腺癌细胞系以及许多其他人类癌细胞系结合。这种杂交瘤抗体命名为10 - 3D2，它不与正常乳腺上皮、多种对照细胞系或选定的正常人组织匀浆结合。10 - 3D2杂交瘤抗体通过免疫沉淀鉴定出一种126千道尔顿的膜蛋白，该蛋白在不同肿瘤细胞中无差异。参照[3H]葡糖胺的掺入情况，它是糖基化的，参照32P的掺入情况，它也是磷酸化的。它以细颗粒状模式存在于乳腺癌细胞的细胞表面，在明显细胞 - 细胞接触部位有浓度聚集。这种126千道尔顿的磷酸糖蛋白，我们一直无法通过对内在标记的正常细胞进行免疫沉淀来鉴定，它可能代表了一种迄今未被认识的人乳腺癌以及某些其他类型人类肿瘤的肿瘤相关标志物。
