Prichard J W, Alger J R, Behar K L, Petroff O A, Shulman R G
Proc Natl Acad Sci U S A. 1983 May;80(9):2748-51. doi: 10.1073/pnas.80.9.2748.
31P NMR studies on the brains of living rabbits were carried out at 32 MHz in a spectrometer having a 200-mm clear bore. Paralyzed pump-ventilated animals under nitrous oxide analgesia were inserted into the 1.89-T field and signals were focused in the brain by using a 4-cm surface coil. Several conventional physiological variables were monitored together with 31P spectra during induction and reversal of insulin shock and hypoxic hypoxia sufficient to abolish the electroencephalogram and during status epilepticus. A reversible decrease in phosphocreatine stores accompanied by an increase in Pi was detected during hypoglycemia and hypoxia. Similar changes were observed in prolonged status epilepticus but were not reversed. ATP levels fell about 50% in hypoglycemia but only slightly in the other two metabolic stresses. Intracellular pH rose in hypoglycemia; in status epilepticus and hypoxia it fell, but only when cardiovascular function was severely impaired. From the measured NMR parameters and the assumptions (i) that creatine kinase was at equilibrium and (ii) that the creatine/phosphocreatine pool was constant, it was possible to calculate the relative changes in cytoplasmic ADP levels associated with these metabolic disturbances.
在一台具有200毫米内径的32兆赫光谱仪上，对活兔大脑进行了31P核磁共振研究。将处于一氧化二氮镇痛下的瘫痪并通过泵进行通气的动物放入1.89特斯拉的磁场中，并使用一个4厘米的表面线圈将信号聚焦在大脑上。在胰岛素休克和足以消除脑电图的低氧性缺氧的诱导和逆转过程中，以及在癫痫持续状态期间，监测了几个常规生理变量以及31P光谱。在低血糖和低氧期间，检测到磷酸肌酸储备可逆性减少，同时无机磷增加。在癫痫持续状态延长期间也观察到类似变化，但未逆转。低血糖时ATP水平下降约50%，但在其他两种代谢应激中仅略有下降。低血糖时细胞内pH值升高；在癫痫持续状态和低氧时，细胞内pH值下降，但仅在心血管功能严重受损时出现。根据测得的核磁共振参数以及（i）肌酸激酶处于平衡状态和（ii）肌酸/磷酸肌酸池恒定的假设，可以计算出与这些代谢紊乱相关的细胞质ADP水平的相对变化。
