Cederblad G, Hickey B E, Hollender A, Akerlund G
Clin Chem. 1978 Jul;24(7):1191-3.
The albumin values determined by the bromcresol green methods do not compare well with values by more specific methods for albumin determination. The discrepancies have been related to, among other things, acute-phase reactants and are especially pronounced in the lower albumin range. These disadvantages are also inherent in a routine continuous-flow method for albumin (SMAC). The bromcresol green method has been improved considerably by shortening the reaction time before the absorbance is measured, as is described here. The modified method yields values that better agree with those by more specific methods and an influence of acute-phase reactants is no longer observed.
用溴甲酚绿法测定的白蛋白值与用更特异的白蛋白测定方法所得的值相比,结果不太理想。这些差异与急性期反应物等因素有关,在白蛋白水平较低时尤为明显。这些缺点在白蛋白的常规连续流动法(SMAC)中也同样存在。如本文所述,通过缩短测量吸光度前的反应时间,溴甲酚绿法已得到显著改进。改进后的方法所得值与更特异方法所得值更相符,且不再观察到急性期反应物的影响。