用离子交换电极法测定正常受试者血清离子钙

Determination of serum ionised calcium by ion-exchange electrode in normal subjects.

作者信息

Conceicao S C, Ward M K, Alvarez-Ude F, Aljama P, Smith P, Kerr D N

出版信息

Clin Chim Acta. 1978 Jun;86(2):143-51. doi: 10.1016/0009-8981(78)90127-4.

Abstract

Serum ionised calcium concentration [Ca2+] was measured with a calcium selective electrode in 65 normal people. A mean value of 1.06 mmol/l (+/- 0.04 S.D.) with an actual range of 0.97 to 1.13 mmol/l was obtained. Serum samples refrigerated at 4 degrees C for 24 h were satisfactory for analysis. Storage of whole blood samples for 6 h at room temperature before separation caused a very small error (+0.04 mmol/l). Mean results and ranges were similar in males and females and there was no significant correlation with age. No significant change in serum [Ca2+] was found following a normal meal. Serum [Ca2+] and total serum calcium showed a very slight correlation (r = 0.35). The method is reproducible and sensitive.

摘要

采用钙选择性电极对65名正常人的血清离子钙浓度[Ca2+]进行了测定。获得的平均值为1.06 mmol/l（±0.04标准差），实际范围为0.97至1.13 mmol/l。在4℃冷藏24小时的血清样本可用于分析。全血样本在分离前于室温下保存6小时会产生非常小的误差（+0.04 mmol/l）。男性和女性的平均结果及范围相似，且与年龄无显著相关性。正常进食后血清[Ca2+]未发现显著变化。血清[Ca2+]与血清总钙呈非常微弱的相关性（r = 0.35）。该方法具有可重复性和敏感性。