Dahl J E
Scand J Dent Res. 1983 Oct;91(5):335-40. doi: 10.1111/j.1600-0722.1983.tb00826.x.
Sixty female Wistar rats were injected with tritiated thymidine and killed at intervals between 1 hr and 72 hr after injection. Autoradiographs of paraffin and Vestopal W embedded sagittal sections of the maxillary incisors were prepared. The distribution of labeled cells after 1 hr revealed the basal pulp cells and the preodontoblasts as proliferative cells. The growth rate of the incisor and the migration of the basal pulp cells were established from the position of labeled cells at 72 hr observation time. The number and localization of labeled and unlabeled mitoses were registered in order to determine the duration of the generation cycle of the proliferative cells. The identical cell generation time of preodontoblasts and basal pulp cells and the migration pattern of the latter indicate that the preodontoblasts are renewed by preodontoblast multiplication and not by migration of basal pulp cells.
给60只雌性Wistar大鼠注射氚标记的胸腺嘧啶核苷，并在注射后1小时至72小时的不同时间点处死。制备了上颌切牙石蜡包埋和Vestopal W包埋矢状切片的放射自显影片。注射1小时后标记细胞的分布显示，牙髓基底细胞和前成牙本质细胞为增殖细胞。根据72小时观察时间点标记细胞的位置确定切牙的生长速率和牙髓基底细胞的迁移情况。记录标记和未标记有丝分裂的数量和定位，以确定增殖细胞的细胞周期持续时间。前成牙本质细胞和牙髓基底细胞相同的细胞生成时间以及后者的迁移模式表明，前成牙本质细胞是通过前成牙本质细胞增殖而更新，而非通过牙髓基底细胞的迁移。
