Dahl J E, Strømme Koppang H
Acta Odontol Scand. 1985 May;43(2):97-102. doi: 10.3109/00016358509046493.
Ten female rats were intravenously injected with doxorubicin (20 mg/kg) and divided into two equal groups receiving a dose of tritiated thymidine either simultaneously with doxorubicin or 1 h before being killed after 5 days. Six control animals were correspondingly injected with 3H-thymidine. The left and right maxillary incisors were prepared for histologic and microautoradiographic investigation, respectively. The distribution of labeled cells in animals injected 1 h before death showed the regions of proliferation, whereas migration from these regions was evaluated by longer observation time. A zone of reduced dentin deposition lined by irregular odontoblasts being young odontoblasts and late preodontoblasts was observed at the time of doxorubicin injection. Pulpally to the dentinal lesion, islands of irregular predentin were deposited by non-progenitive pulp cells and depolarized odontoblasts. The late preodontoblasts were renewed from progenitive pulp cells, leading to a disturbed mantle predentin deposition.
将十只雌性大鼠静脉注射阿霉素（20毫克/千克），并分为两组，每组五只，一组在注射阿霉素的同时给予一定剂量的氚标记胸腺嘧啶核苷，另一组在5天后处死前1小时给予该物质。相应地，给六只对照动物注射³H-胸腺嘧啶核苷。分别对左右上颌切牙进行组织学和显微放射自显影研究。在死亡前1小时注射的动物中，标记细胞的分布显示了增殖区域，而通过更长的观察时间评估了这些区域的细胞迁移情况。在注射阿霉素时，观察到一个牙本质沉积减少的区域，其内侧为不规则的成牙本质细胞，这些细胞为年轻的成牙本质细胞和晚期前成牙本质细胞。在牙髓侧的牙本质病变处，非增殖性牙髓细胞和去极化的成牙本质细胞沉积了不规则前期牙本质岛。晚期前成牙本质细胞由增殖性牙髓细胞更新，导致罩牙本质沉积紊乱。
