Goodson J M, Holborow D, Dunn R L, Hogan P, Dunham S
J Periodontol. 1983 Oct;54(10):575-9. doi: 10.1902/jop.1983.54.10.575.
For the purpose of developing controlled delivery devices for periodontal therapy which would release over several days, six fiber types made of tetracycline-loaded biocompatible polymers were manufactured and tested. Polyethylene, polypropylene, polycaprolactone, polyurethane and cellulose acetate propionate all released their drug load within 1 day. Ethylene vinyl acetate fibers, however, provided in vitro sustained release for periods up to 9 days. A bioassay was designed to measure levels of tetracycline achieved by local delivery which used growth inhibition of Bacillus cereus as a measure of the amount of tetracycline in measured volumes of gingival sulcus fluid on filter paper strips. By this assay, fibers made from 25% loaded ethylene vinyl acetate established initial concentrations of approximately 500 micrograms/ml. The measurement of tetracycline concentration resulting from the placement of these fibers into deep periodontal pockets as a packing material provided indication that concentrations of greater than 50 micrograms/ml could be maintained for months by weekly to monthly replacement. These studies indicate that monolithic fibers made of tetracycline-loaded ethylene vinyl acetate have characteristics which could prove useful as the basis of a tetracycline delivery system for the treatment of periodontal disease.
为了开发用于牙周治疗的控释装置，使其能在数天内持续释放药物，制备并测试了六种由负载四环素的生物相容性聚合物制成的纤维类型。聚乙烯、聚丙烯、聚己内酯、聚氨酯和醋酸丙酸纤维素在1天内就释放了全部药物。然而，乙烯-醋酸乙烯酯纤维在体外可持续释放长达9天。设计了一种生物测定法来测量局部给药后四环素的水平，该方法利用蜡样芽孢杆菌的生长抑制作为滤纸条上龈沟液测量体积中四环素含量的指标。通过该测定法，由负载25%四环素的乙烯-醋酸乙烯酯制成的纤维建立了约500微克/毫升的初始浓度。将这些纤维作为填充材料放入牙周深袋后对四环素浓度的测量表明，通过每周至每月更换，浓度大于50微克/毫升可维持数月。这些研究表明，由负载四环素的乙烯-醋酸乙烯酯制成的整体纤维具有的特性，可作为治疗牙周疾病的四环素给药系统基础，具有实用价值。
