Sasaki T
Arch Oral Biol. 1983;28(10):895-905. doi: 10.1016/0003-9969(83)90084-5.
Glutaraldehyde-fixed rat incisors were either post-fixed in ferrocyanide-reduced osmium impregnated with the Ur-Pb-Cu technique or incubated in the medium for acid-phosphatase (AcPase) reaction. The Golgi apparatus of the secretory ameloblast was composed of 4-7 cisternae, small vesicles and condensing vacuoles. It formed an elongated, continuous membrane system over from one- to two-thirds of the supranuclear cytoplasm. Condensing vacuoles seemed to be produced from the dilated margins of both Golgi cisternae and GERL. AcPase activity was demonstrated in the inner 2 or 3 Golgi cisternae, in GERL and in some condensing vacuoles and secretory granules within the Tomes process. It thus seemed that primary lysosomes originate from both the Golgi apparatus and GERL. Whereas many lysosomal bodies appeared in the supranuclear cytoplasm, autophagic vacuoles were rare. A well-developed Golgi apparatus and GERL were, therefore, considered to be involved in the digestion of exogeneous materials as well as in the formation of the precursor of enamel matrix. The simple Golgi apparatus consisting of only compactly stacked cisternae and small vesicles, were sometimes observed in the supranuclear cytoplasm and having no clear relationship with rough-surfaced endoplasmic reticula or GERL, may serve as a source of the plasma membranes necessary for continuous renewal of the cisternae of the well-developed Golgi apparatus.
戊二醛固定的大鼠切牙，要么用亚铁氰化物还原锇浸渍的Ur-Pb-Cu技术进行后固定，要么在酸性磷酸酶（AcPase）反应的培养基中孵育。分泌性成釉细胞的高尔基体由4-7个扁平囊、小泡和浓缩泡组成。它在核上细胞质的三分之一到三分之二的范围内形成一个细长的、连续的膜系统。浓缩泡似乎是由高尔基体扁平囊和GERL的扩张边缘产生的。在高尔基体内部的2或3个扁平囊、GERL以及托姆斯突内的一些浓缩泡和分泌颗粒中都显示出AcPase活性。因此，初级溶酶体似乎起源于高尔基体和GERL。虽然在核上细胞质中出现了许多溶酶体，但自噬泡很少见。因此，发育良好的高尔基体和GERL被认为参与了外源物质的消化以及釉质基质前体的形成。有时在核上细胞质中观察到仅由紧密堆叠的扁平囊和小泡组成的简单高尔基体，且与糙面内质网或GERL没有明显关系，它可能是发育良好的高尔基体扁平囊持续更新所需质膜的来源。
