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关于干燥但仍具活力细胞的残余含水量

On the residual water content of dried but viable cells.

作者信息

Clegg J S, Zettlemoyer A C, Hsing H H

出版信息

Experientia. 1978 Jun 15;34(6):734. doi: 10.1007/BF01947290.

DOI:10.1007/BF01947290
PMID:658284
Abstract

We have examined the residual water in dried cysts of the brine shrimp, Artemia salina, by gas-bombardment techniques at reduced pressures and temperatures. This treatment reduced, but did not remove all the residual water, the lower limit being about 0.0069 gH2O/g dried weight. The significance of such small amounts of water to cell hydration is assessed. The treatment did not appreciably reduce the viability of this cyst population.

摘要

我们通过在减压和低温条件下的气体轰击技术,检测了卤虫(Artemia salina)干燥囊肿中的残留水分。这种处理减少了,但并未去除所有残留水分,下限约为0.0069 gH₂O/ g干重。评估了如此少量的水对细胞水合作用的意义。该处理并未明显降低这群囊肿的活力。

相似文献

1
On the residual water content of dried but viable cells.关于干燥但仍具活力细胞的残余含水量
Experientia. 1978 Jun 15;34(6):734. doi: 10.1007/BF01947290.
2
Interrelationships between water and cellular metabolism in Artemia cysts. IV. Adenosine 5'-triphosphate and cyst hydration.卤虫休眠卵中水与细胞代谢的相互关系。IV. 腺苷5'-三磷酸与休眠卵水合作用
J Cell Physiol. 1976 Jun;88(2):159-66. doi: 10.1002/jcp.1040880205.
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Interrelationships between Water and cellular metabolism in Artemia cysts. VIII Sorption isotherms and derived thermodynamic quantities.卤虫休眠卵中水与细胞代谢的相互关系。VIII吸附等温线及导出的热力学量。
J Cell Physiol. 1978 Feb;94(2):123-37. doi: 10.1002/jcp.1040940202.
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Interrelationships between water and cellular metabolism in Artemia cysts. VII. Free amino acids.卤虫休眠卵中水与细胞代谢的相互关系。VII. 游离氨基酸。
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Interrelationships between water and cellular metabolism in Artemia cysts. V. 14CO2 incorporation.卤虫休眠卵中水与细胞代谢的相互关系。V. 14CO₂掺入
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A gas chromatographic method of measuring residual water in freeze-dried smallpox vaccine.一种测定冻干天花疫苗中残留水分的气相色谱法。
Bull World Health Organ. 1972;47(1):7-11.
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Interrelationships between water and cellular metabolism in Artemia cysts. VI. RNA and protein synthesis.卤虫卵中水与细胞代谢的相互关系。VI. RNA与蛋白质合成。
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The lipids of Mono Lake, California, brine shrimp (Artemia salina).
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Studies on residual moisture of freeze-dried biological products. I. Comparative evaluation of some methods for establishing water content of freeze-dried specimens.冻干生物制品残留水分的研究。I. 确定冻干样品含水量的一些方法的比较评估。
Arch Roum Pathol Exp Microbiol. 1988 Apr-Jun;47(2):145-55.
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Methodology for in-process determination of residual water in freeze-dried products.冻干产品中残留水分过程中测定的方法学。
Dev Biol Stand. 1992;74:137-50; dicussion 150-1.

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Cell Stress Chaperones. 2016 Jan;21(1):139-154. doi: 10.1007/s12192-015-0647-3.
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Quantification of cellular protein expression and molecular features of group 3 LEA proteins from embryos of Artemia franciscana.卤虫胚胎中第3组胚胎发育晚期丰富蛋白(LEA蛋白)的细胞蛋白表达及分子特征的定量分析
Cell Stress Chaperones. 2014 May;19(3):329-41. doi: 10.1007/s12192-013-0458-3. Epub 2013 Sep 6.
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Diffusive properties of water in Artemia cysts as determined from quasi-elastic neutron scattering spectra.

本文引用的文献

1
Drying of living mammalian cells.
Ann N Y Acad Sci. 1960 Apr 13;85:729-34. doi: 10.1111/j.1749-6632.1960.tb49993.x.
2
The problem of anabiosis or latent life: history and current concept.隐生现象或潜在生命问题:历史与当前概念
Proc R Soc Lond B Biol Sci. 1959 Mar 17;150(939):149-91. doi: 10.1098/rspb.1959.0013.
3
Stabilities of dried suspensions of influenza virus sealed in a vacuum or under different gases.密封在真空或不同气体环境下的流感病毒干燥悬浮液的稳定性。
Appl Microbiol. 1969 Jun;17(6):830-5. doi: 10.1128/am.17.6.830-835.1969.
由准弹性中子散射光谱测定卤虫卵囊中水分的扩散特性。
Biophys J. 1984 May;45(5):927-38. doi: 10.1016/S0006-3495(84)84239-3.
4
Sorption of water vapour and nitrogen gas by bacterial spores.细菌芽孢对水蒸气和氮气的吸附作用。
Nature. 1967 Dec 30;216(5122):1304-6. doi: 10.1038/2161304a0.
5
Interrelationships between water and metabolism in Artemia salina cysts: hydration-dehydration from the liquid and vapour phases.卤虫囊肿中水与代谢的相互关系:液相和气相的水合-脱水作用
J Exp Biol. 1974 Oct;61(2):291-308. doi: 10.1242/jeb.61.2.291.