Bai G Z, Hara H, Nagai K
Exp Hematol. 1984 Mar;12(3):171-6.
To investigate the influence of nonlymphocytic leukemic cells on normal erythropoietic burst-forming units (BFU-E), we cocultured leukemic cells at concentrations ranging from 0.5 to 5.0 X 10(5)/ml with 2 X 10(5) blood mononuclear cells or marrow nucleated cells from normal subjects in a BFU-E assay. The inhibitory effect of leukemic cells on burst formation by normal BFU-E was detected only at a high concentration (5 X 10(5)/ml). Leukemic-cell-conditioned medium (LCCM) was prepared by adding 1.25-10 X 10(6)/ml leukemic cells to culture medium. LCCM caused a dose-dependent inhibition of burst formation by normal BFU-E, but the medium prepared from normal cells did not. To examine the influence of fluoxymesterone (FM) on the inhibition of burst formation, we added 10(-10)-10(-7) M FM to the culture medium. Inhibition of burst formation by normal BFU-E was reduced from 73.4% to 58.2%-63.1%. These results suggest that FM stimulates proliferation and differentiation of normal BFU-E that are affected by inhibitory factors produced by leukemic cells.
为了研究非淋巴细胞白血病细胞对正常红细胞爆式集落形成单位（BFU-E）的影响，我们在BFU-E检测中，将浓度范围为0.5至5.0×10⁵/ml的白血病细胞与来自正常受试者的2×10⁵个血液单核细胞或骨髓有核细胞进行共培养。仅在高浓度（5×10⁵/ml）时才检测到白血病细胞对正常BFU-E爆式集落形成的抑制作用。白血病细胞条件培养基（LCCM）是通过向培养基中添加1.25 - 10×10⁶/ml白血病细胞制备而成。LCCM对正常BFU-E的爆式集落形成产生剂量依赖性抑制，但由正常细胞制备的培养基则无此作用。为了研究氟甲睾酮（FM）对爆式集落形成抑制作用的影响，我们向培养基中添加了10⁻¹⁰ - 10⁻⁷M的FM。正常BFU-E的爆式集落形成抑制率从73.4%降至58.2% - 63.1%。这些结果表明，FM可刺激受白血病细胞产生的抑制因子影响的正常BFU-E的增殖和分化。
