School of Medicine and Dentistry, Santiago de Compostela University, Calle Entrerrios s/n, Santiago de Compostela 15782, Spain.
Clin Oral Investig. 2010 Aug;14(4):397-402. doi: 10.1007/s00784-009-0320-2. Epub 2009 Aug 8.
The in vivo antimicrobial activity of 0.12% and 0.2% chlorhexidine (CHX) on the salivary flora up to 7 h after its application, using epifluorescence microscopy with the SYTO 9/propidium iodide dual staining, was evaluated. Fifteen volunteers performed a single mouthrinse with sterile water (SM-water), a single mouthrinse with 0.12% CHX (0.12% SM-CHX) and a single and double mouthrinse with 0.2% CHX (0.2% SM-CHX and 0.2% DM-CHX). Samples of saliva were taken at 30 s, and 1, 3, 5, and 7 h after each application. In comparison with SM-water, 0.2% CHX (SM and DM) showed a significant antibacterial effect up to 7 h after the mouthrinse, whereas this effect only persisted up to 5 h after the 0.12% SM-CHX mouthrinse. On comparing the two concentrations of CHX, significantly higher percentages of bacterial vitality were observed in all the saliva samples after the use of 0.12% CHX than after 0.2% CHX. On comparison of the 0.2% SM-CHX and 0.2% DM-CHX, significantly higher percentages of live bacteria were observed in the saliva samples taken at 1, 3, 5, and 7 h after the single mouthrinse compared with the double mouthrinse. The 0.2% CHX mouthrinse had the greatest antimicrobial activity on the salivary flora up to 7 h after its application, with a progressive recovery in bacterial vitality. The differences observed with respect to the 0.12% CHX mouthrinse demonstrate the influence of the concentration on its immediate antimicrobial activity and substantivity.
采用 SYTO 9/碘化丙啶双重染色的荧光显微镜法，评估了应用 0.12%和 0.2%洗必泰（CHX）后 7 小时内其对唾液菌群的体内抗菌活性。15 名志愿者分别使用无菌水（SM-水）、0.12% CHX（0.12% SM-CHX）进行单次漱口，以及 0.2% CHX（0.2% SM-CHX 和 0.2% DM-CHX）进行单次和双次漱口。在每次应用后 30s 及 1、3、5 和 7h 采集唾液样本。与 SM-水相比，0.2% CHX（SM 和 DM）在漱口后 7h 内表现出显著的抗菌作用，而 0.12% SM-CHX 漱口后仅能持续 5h。比较两种浓度的 CHX，在使用 0.12% CHX 后所有唾液样本中观察到的细菌活力百分比均显著高于使用 0.2% CHX 后。在比较 0.2% SM-CHX 和 0.2% DM-CHX 时，与双次漱口相比，单次漱口后 1、3、5 和 7h 采集的唾液样本中活细菌的百分比明显更高。0.2% CHX 漱口在应用后 7 小时内对唾液菌群具有最大的抗菌活性，且细菌活力呈逐渐恢复趋势。与 0.12% CHX 漱口相比，观察到的差异表明，浓度对其即刻抗菌活性和持续性有影响。
