神经元细胞悬液的脑内移植。I. 引言及一般制备方法。
Intracerebral grafting of neuronal cell suspensions. I. Introduction and general methods of preparation.
作者信息
Björklund A, Stenevi U, Schmidt R H, Dunnett S B, Gage F H
出版信息
Acta Physiol Scand Suppl. 1983;522:1-7.
PMID:6586054
Abstract
The steps involved in the grafting of mesencephalic and septal embryonic tissue in the form of dissociated cell suspensions are described in detail. This includes dissection of the donor embryos, incubation in trypsin, mechanical dissociation, and stereotaxic injection into the brains of adult recipient rats. Some of the technical problems and limitations are discussed.
摘要
详细描述了以解离细胞悬液形式移植中脑和隔区胚胎组织所涉及的步骤。这包括供体胚胎的解剖、胰蛋白酶孵育、机械解离以及立体定向注射到成年受体大鼠的大脑中。还讨论了一些技术问题和局限性。
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