Hawkins C C, Buggy B P, Fekety R, Schaberg D R
J Infect Dis. 1984 May;149(5):775-80. doi: 10.1093/infdis/149.5.775.
The epidemiology of colitis induced by Clostridium difficile in hamsters was studied with a new bacteriophage and bacteriocin typing system. Fatal enterocolitis was induced by administration of N-formimidoyl thienamycin. Environmental cultures were obtained repeatedly throughout the experiments. Thirteen percent of 90 healthy hamsters were already colonized with C difficile on arrival from the supplier. Mortality from enterocolitis after antibiotic administration was 75% and was not diminished by use of a laminar-flow facility. The same uncommon bacteriocin type (83/1309/2329) of toxigenic C difficile that colonized hamsters on arrival was recovered from the cecal contents of all hamsters dying with enterocolitis and from most environmental isolates. Previously uncolonized , antibiotic-treated hamsters placed into cages where animals had died from enterocolitis also developed enterocolitis with the same bacteriocin type (83/1309/2329), an outcome suggesting acquisition of C difficile from the environment.
利用一种新的噬菌体和细菌素分型系统，对仓鼠艰难梭菌诱导的结肠炎流行病学进行了研究。通过给予N-甲酰亚胺硫霉素诱导致死性小肠结肠炎。在整个实验过程中反复获取环境培养物。从供应商处运来的90只健康仓鼠中，13%在到达时就已被艰难梭菌定植。抗生素给药后小肠结肠炎的死亡率为75%，使用层流设施并未降低死亡率。所有死于小肠结肠炎的仓鼠盲肠内容物以及大多数环境分离株中，均检出了与到达时定植仓鼠的产毒艰难梭菌相同的罕见细菌素型（83/1309/2329）。将先前未定植、经抗生素处理的仓鼠放入动物死于小肠结肠炎的笼子中，这些仓鼠也会发生相同细菌素型（83/1309/2）的小肠结肠炎，这一结果表明艰难梭菌是从环境中获得的。
