Giulian D
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3567-71. doi: 10.1073/pnas.81.11.3567.
Severed ganglion cell axons of the goldfish retina regrow and form new synaptic connections within their primary target tissue, the optic tectum. During axonal regeneration, optic tract glia show increased incorporation of both thymidine and amino acids. Ablation of the tectum reduces the biosynthetic activity of cells in the optic tract, suggesting that humoral factors released from the tectum may stimulate neighboring glia. A soluble fraction isolated from denervated tecta increases glial incorporation of both thymidine and amino acids by 2- to 3-fold when compared to control cells treated with soluble material from intact tecta. One glial promoting factor, designated GPF -1, is a trypsin-sensitive peptide of about 14,000 daltons. Peptides released from target tissues may help to regulate growth of glial cells during neuronal regeneration.
金鱼视网膜中被切断的神经节细胞轴突能够再生，并在其主要靶组织——视顶盖内形成新的突触连接。在轴突再生过程中，视束胶质细胞显示出胸苷和氨基酸掺入量增加。顶盖切除会降低视束中细胞的生物合成活性，这表明从顶盖释放的体液因子可能刺激邻近的胶质细胞。与用完整顶盖的可溶性物质处理的对照细胞相比，从去神经支配的顶盖中分离出的可溶性组分可使胶质细胞的胸苷和氨基酸掺入量增加2至3倍。一种被称为GPF -1的胶质促进因子是一种分子量约为14,000道尔顿的对胰蛋白酶敏感的肽。从靶组织释放的肽可能有助于在神经元再生过程中调节胶质细胞的生长。
