Hume W R
J Dent Res. 1984 Jun;63(6):881-4. doi: 10.1177/00220345840630061301.
Tritium-labeled eugenol was released from mixtures of zinc oxide eugenol (ZOE) into aqueous solution at rates which declined exponentially with time, and which were directly proportional to the liquid-powder ratio. The release pattern was consistent with a model of progressive hydrolysis of zinc eugenolate in a limited-thickness ZOE surface layer. Intervening dentin had a profound effect on this pattern of release. In human teeth in vitro containing ZOE as a base or temporary filling, peak eugenol release at the pulpal surface of dentin was of the order of a thousand-fold less than that at the salivary surface. In such teeth, eugenol reached concentrations in excess of 10(-2) M in dentin just beneath ZOE, and 10(-4) M or less adjacent to the pulp space. Both pulpal outflow and dentin concentrations of eugenol remained relatively constant for more than a week, unlike release into aqueous solution. While these data were derived from studies on human teeth in vitro, they give a strong indication of probable events in vivo, and appear to provide a basis for the explanation of the paradox of the therapeutic and toxic actions of ZOE.
氚标记的丁香酚从氧化锌丁香酚（ZOE）混合物中以随时间呈指数下降的速率释放到水溶液中，且该速率与液粉比成正比。释放模式与丁香酚锌在有限厚度的ZOE表层中进行性水解的模型一致。中间的牙本质对这种释放模式有深远影响。在体外含有ZOE作为基底或临时充填物的人牙中，牙本质牙髓表面的丁香酚释放峰值比唾液表面的释放峰值低约一千倍。在这类牙齿中，丁香酚在紧邻ZOE下方的牙本质中达到超过10⁻² M的浓度，而在靠近牙髓腔处浓度为10⁻⁴ M或更低。与释放到水溶液中不同，丁香酚的牙髓流出量和牙本质浓度在一周多的时间里都保持相对恒定。虽然这些数据来自对人牙的体外研究，但它们有力地表明了体内可能发生的事件，并且似乎为解释ZOE治疗作用和毒性作用的矛盾提供了基础。
