Hume W R
J Dent Res. 1984 Nov;63(11):1262-5. doi: 10.1177/00220345840630110101.
Eugenol depressed cell respiration in homogenates of human dental pulp and in mouse fibroblast monolayers. The depression was concentration-dependent, with a threshold at about 10(-4)M and a maximum at 10(-3)M in both preparations. Onset of the depression appeared to be rapid. The effects of variation in both duration and concentration of eugenol exposure on subsequent uptake of 3H-thymidine were examined in mouse fibroblast monolayers and human pulp explants. Fibroblasts survived short-term (up to 12 hr) exposure to 10(-3)M eugenol or less, but died after exposure to 10(-3)M for one day or more. The cells survived exposure to 10(-4)M for ten days, the longest period examined. Human pulp maintained in tissue culture medium showed similar eugenol susceptibility. Analysis of these data, when coupled with those of previous studies on eugenol release from ZOE and diffusion through dentin, gives strong support for the concepts that: the blandness of ZOE when applied to intact dentin is due to eugenol reaching the pulp in sub-toxic concentrations, and the irritant effect of ZOE when applied directly to soft tissue is due to the development of concentrations of eugenol in tissue adjacent to ZOE sufficient to inhibit respiration and thus kill cells.
丁香酚可抑制人牙髓匀浆和小鼠成纤维细胞单层的细胞呼吸。这种抑制作用呈浓度依赖性，在两种制剂中，阈值约为10⁻⁴M，最大抑制浓度为10⁻³M。抑制作用的开始似乎很快。在小鼠成纤维细胞单层和人牙髓外植体中，研究了丁香酚暴露的持续时间和浓度变化对随后3H-胸腺嘧啶摄取的影响。成纤维细胞在短期（长达12小时）暴露于10⁻³M或更低浓度的丁香酚中存活，但在暴露于10⁻³M丁香酚一天或更长时间后死亡。细胞在暴露于10⁻⁴M丁香酚的情况下存活了十天，这是所研究的最长时间。维持在组织培养基中的人牙髓表现出类似的丁香酚敏感性。将这些数据与先前关于丁香酚从氧化锌丁香酚水门汀释放并扩散通过牙本质的研究数据相结合，有力地支持了以下观点：当应用于完整牙本质时，氧化锌丁香酚水门汀的温和性是由于丁香酚以亚毒性浓度到达牙髓，而当直接应用于软组织时，氧化锌丁香酚水门汀的刺激作用是由于在氧化锌丁香酚水门汀附近组织中丁香酚浓度升高，足以抑制呼吸并因此杀死细胞。
