Cosentino M J, Takihara H, Burhop J W, Cockett A T
J Androl. 1984 May-Jun;5(3):216-22. doi: 10.1002/j.1939-4640.1984.tb02399.x.
Mechanical activity of the rat caput epididymidis in vitro was recorded using a videomicrography system. The effects of prostaglandin (PG)F2 alpha, PGE2, and aspirin on caput epididymidis contractility were determined by measuring the frequency of contraction, luminal diameter, and amplitude of contraction at various concentrations of each test compound in vitro. PGF2 alpha stimulated contractility of the tubules at physiological concentrations, while PGE2 reduced contractility. Aspirin strongly inhibited contractility at concentrations of 10(-3) and 10(-2)M. Endogenous levels of PGF2 alpha and PGE were determined for rat testes, caput, corpus, and cauda epididymidis and vas deferens. While the concentrations of PGE were consistently higher than those of PGF2 alpha, both compounds were relatively low in the testes, high in the vas deferens, and intermediate throughout the epididymis. Results from these experiments strongly suggest that PGs are important regulators of proximal epididymidis contractions and thus may regulate sperm transport through that organ.
使用视频显微摄像系统记录大鼠附睾头的体外机械活动。通过在体外不同浓度的每种测试化合物下测量收缩频率、管腔直径和收缩幅度，来确定前列腺素（PG）F2α、PGE2和阿司匹林对附睾头收缩性的影响。PGF2α在生理浓度下刺激小管的收缩性，而PGE2则降低收缩性。阿司匹林在10^(-3)和10^(-2)M浓度下强烈抑制收缩性。测定了大鼠睾丸、附睾头、体、尾以及输精管中PGF2α和PGE的内源性水平。虽然PGE的浓度始终高于PGF2α，但这两种化合物在睾丸中相对较低，在输精管中较高，而在整个附睾中处于中间水平。这些实验结果强烈表明，前列腺素是附睾近端收缩的重要调节因子，因此可能调节精子通过该器官的运输。
