Maizel A, Mehta S, Kouttab N, Morgan J, Ford R, Sahasrabuddhe C
Fed Proc. 1983 Jul;42(10):2753-6.
Recent studies have established the ability of a proportion of activated human B lymphocytes to undergo G1 phase cell cycle progression and subsequent S phase entry on exposure to factor(s) present in lectin-stimulated mononuclear cell-conditioned media. One factor capable of stimulating activated human B lymphocyte proliferation may be separated from peripheral blood lymphocyte-conditioned media by successive ammonium sulfate precipitation, ion exchange, and gel filtration chromatography. The isolated factor is distinct from the other well-described cytokines, possesses a molecular weight of 12,000-13,000, has a mildly acidic isoelectric point (at pH 6.3-6.6), is protease sensitive, and is relatively heat sensitive. The human B cell mitogenic factor possesses functional and cellular specificity in that its action is restricted to B lymphocytes and its function is proliferative. The production of the B cell mitogenic factor by T lymphocytes is augmented by the presence of a macrophage and further stimulated by syngeneic B cells.
最近的研究证实，一部分活化的人B淋巴细胞在接触凝集素刺激的单核细胞条件培养基中存在的因子后，能够经历G1期细胞周期进程并随后进入S期。一种能够刺激活化的人B淋巴细胞增殖的因子可通过连续的硫酸铵沉淀、离子交换和凝胶过滤色谱从外周血淋巴细胞条件培养基中分离出来。分离出的因子与其他已充分描述的细胞因子不同，分子量为12,000 - 13,000，具有轻度酸性的等电点（pH 6.3 - 6.6），对蛋白酶敏感，且相对热敏感。人B细胞促有丝分裂因子具有功能和细胞特异性，因为其作用仅限于B淋巴细胞且其功能是增殖性的。T淋巴细胞产生B细胞促有丝分裂因子会因巨噬细胞的存在而增强，并受到同基因B细胞的进一步刺激。
