Wyczółkowska J, Brzezińska-Błaszczyk E, Maśliński C
Int Arch Allergy Appl Immunol. 1983;72(1):16-21. doi: 10.1159/000234834.
The IgE antibody and total IgE responses were studied in three mouse strains immunized with low doses of ovalbumin. BALB/c and C57BL/6J mice were considered as 'high' and 'intermediate' IgE antibody responders, respectively; during immunization the peritoneal mast cells of mice from these two strains became sensitized. In identical conditions of immunization, very low IgE antibody response and lack of mast cell sensitization was observed in CFW mice. After exposure of these CFW mice to low-dose X-irradiation (250 R) or a moderate dose of cyclophosphamide (100 mg/kg), a detectable IgE antibody response appeared and peritoneal mast cells became sensitized. In all three strains the evolution of total serum IgE levels during immunization did not correlate with IgE antibody response; an early transient elevation of total IgE level appeared soon after first immunization, at a time when IgE antibody responses were not detectable or were very low. After X-irradiation or cyclophosphamide pretreatment, this elevation of total IgE was potentiated. The possibility of differences in regulation of specific IgE antibody response and IgE of unknown specificity in the mice is discussed.
对用低剂量卵清蛋白免疫的三种小鼠品系的IgE抗体和总IgE反应进行了研究。BALB/c和C57BL/6J小鼠分别被视为IgE抗体“高”反应者和“中”反应者；在免疫过程中，这两个品系小鼠的腹膜肥大细胞被致敏。在相同的免疫条件下，CFW小鼠的IgE抗体反应非常低，且未出现肥大细胞致敏。在用低剂量X射线（250 R）或中等剂量环磷酰胺（100 mg/kg）处理这些CFW小鼠后，出现了可检测到的IgE抗体反应，腹膜肥大细胞也被致敏。在所有三个品系中，免疫过程中血清总IgE水平的变化与IgE抗体反应无关；首次免疫后不久，总IgE水平会出现早期短暂升高，此时IgE抗体反应无法检测到或非常低。经X射线照射或环磷酰胺预处理后，总IgE的这种升高会增强。文中讨论了小鼠中特异性IgE抗体反应和未知特异性IgE调节存在差异的可能性。
