Zhuravkov A L, Iliushin V A, Maianskiĭ A N
Zh Mikrobiol Epidemiol Immunobiol. 1983 May(5):78-81.
The stimulating action of the mitogenically active S. aureus metabolite (MASAM), described in earlier works, in the cultures of human peripheral blood lymphocytes, both nonfractionated and separated into T- and B-fractions, as well as the effect of macrophage removal on the sensitivity of lymphocytes to MASAM, were studied. MASAM was found to stimulate T- and B-lymphocyte cultures. The removal of phagocytic cells by means of carbonyl iron resulted in the enhanced sensitivity of nonfractionated lymphocyte cultures to MASAM, inhibited the response of T-lymphocytes to MASAM and had no effect on the sensitivity of B-lymphocyte cultures to the mitogen under study. The comparison of the effect produced on lymphocyte cultures by MASAM and by the commonly used mitogen, phytohemagglutinin, demonstrated that after the removal of macrophages T-lymphocyte cultures lost their sensitivity to MASAM to a greater degree than their sensitivity to phytohemagglutinin. The possible causes of these effects are discussed.
研究了早期文献中描述的有丝分裂原活性金黄色葡萄球菌代谢产物（MASAM）对人外周血淋巴细胞培养物（包括未分离的以及分离为T细胞和B细胞组分的培养物）的刺激作用，以及巨噬细胞去除对淋巴细胞对MASAM敏感性的影响。发现MASAM可刺激T淋巴细胞和B淋巴细胞培养物。通过羰基铁去除吞噬细胞导致未分离的淋巴细胞培养物对MASAM的敏感性增强，抑制T淋巴细胞对MASAM的反应，并且对B淋巴细胞培养物对所研究有丝分裂原的敏感性没有影响。对MASAM和常用有丝分裂原植物血凝素对淋巴细胞培养物产生的作用进行比较表明，去除巨噬细胞后，T淋巴细胞培养物对MASAM的敏感性丧失程度大于对植物血凝素的敏感性丧失程度。讨论了这些作用的可能原因。
