流感嗜血杆菌在添加了血红素和烟酰胺腺嘌呤二核苷酸的模拟血培养物中的生长情况。
Growth of Haemophilus influenzae in simulated blood cultures supplemented with hemin and NAD.
作者信息
Artman M, Domenech E, Weiner M
出版信息
J Clin Microbiol. 1983 Aug;18(2):376-9. doi: 10.1128/jcm.18.2.376-379.1983.
Abstract
The mean generation time of Haemophilus influenzae in simulated blood cultures is 103 to 107 min. With 0.56 to 0.58 doublings per h, even large inocula of 256 cells per ml reach only 2 X 10(6) cells per ml and produce no visible evidence of growth after 24 h of incubation. Hemin and NAD added to simulated blood cultures triple the rate of growth of H. influenzae, so that even small inocula produce visible turbidity after overnight incubation. With a mean generation time of 36 min, a single cell of H. influenzae in simulated blood culture supplemented with hemin and NAD undergoes 30 doublings in 18 h, producing 2(30) (1.07 X 10(9] cells and visible turbidity.
摘要
流感嗜血杆菌在模拟血培养中的平均代时为103至107分钟。每小时倍增0.56至0.58次，即使每毫升接种256个细胞的大接种量，在培养24小时后也仅达到每毫升2×10(6)个细胞，且没有可见的生长迹象。添加到模拟血培养中的血红素和烟酰胺腺嘌呤二核苷酸使流感嗜血杆菌的生长速率提高两倍，因此即使是小接种量在过夜培养后也会产生可见的浑浊。平均代时为36分钟，在添加了血红素和烟酰胺腺嘌呤二核苷酸的模拟血培养中，一个流感嗜血杆菌细胞在18小时内经历30次倍增，产生2(30)(1.07×10(9)个细胞并出现可见浑浊。
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