Weinberg S R, Macvittie T J, Bakarich A C, McGarry M P
Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Oct;44(4):367-75. doi: 10.1080/09553008314551311.
On day 33 of gestation, foetal beagles were irradiated in utero (0.9 Gy of 60Co gamma-irradiation, 0.4 Gy/min). Foetal haematocytopoiesis was studied during the third trimester of gestation (days 42-55). Peripheral blood nucleated cell counts were 33 per cent lower than normal on day 44 and continued to be lower until day 49, when values became higher than normal. Splenic cellularities of irradiated pups on day 44 were more than 3 times those of the nonirradiated, but thereafter they were similar to normal. Differences in haemopoietic progenitor cell activity between irradiated and normal foetuses were observed. In comparison with the other foetal tissues, the foetal liver appeared to experience greater radiation injury. For example, on day 44, the irradiated liver BFU-E, CFU-E, and GM-CFC per 10(5) cells were almost fivefold lower than normal values. Spleens of irradiated foetal beagles contained a marked increase in all haemopoietic progenitor cells (BFU-E, CFU-E, and GM-CFC) and recognizable proliferative granulocytic cells and nucleated erythroid cells. The haemopoietic activity of the irradiated bone marrow during days 42-44 was similar to that of the irradiated spleen, and compensated for the damaged liver. However, unlike the irradiated spleen, the irradiated bone marrow had decreased BFU-E activity compared with the values for the nonirradiated bone marrow during days 48-55. Until day 50, the irradiated marrow contained fewer recognizable proliferative granulocytic cells but more nucleated erythroid cells.
在妊娠第33天，对胎犬进行子宫内照射（60Coγ射线照射，剂量为0.9 Gy，照射速率为0.4 Gy/min）。在妊娠晚期（第42 - 55天）研究胎犬的血细胞生成情况。第44天外周血有核细胞计数比正常水平低33%，并持续降低直至第49天，此后高于正常水平。第44天受照射幼犬的脾脏细胞数量是未受照射幼犬的3倍多，但此后与正常情况相似。观察到受照射胎儿与正常胎儿造血祖细胞活性存在差异。与其他胎儿组织相比，胎儿肝脏似乎受到了更严重的辐射损伤。例如，在第44天，每10(5)个受照射肝脏细胞中的BFU - E、CFU - E和GM - CFC几乎比正常值低五倍。受照射胎犬的脾脏中所有造血祖细胞（BFU - E、CFU - E和GM - CFC）显著增加，且有可识别的增殖性粒细胞和有核红细胞。在第42 - 44天，受照射骨髓的造血活性与受照射脾脏相似，可代偿受损的肝脏。然而，与受照射脾脏不同的是，在第48 - 55天，受照射骨髓的BFU - E活性与未受照射骨髓相比有所降低。直到第50天，受照射骨髓中可识别的增殖性粒细胞较少，但有核红细胞较多。
