Levine S, Sowinski R, Koulish S
Exp Mol Pathol. 1983 Dec;39(3):364-76. doi: 10.1016/0014-4800(83)90065-5.
Two intravenous inoculations of metallic tin powder caused a striking, self-limited enlargement of the spleen up to five or six times its normal size. In addition to epithelioid cell granulomas in response to the foreign particles, the spleen contained extensive accumulations of plasmablasts, plasma cells, and plasma cells containing Russell bodies. The proliferative activity of the immature plasma cells was indicated by the high incidence of mitoses and cells labeled with tritiated thymidine. In contrast, the granulomas had no mitoses or labeling. A single intravenous injection of tin usually produced little or no reaction in the spleen despite an abundance of tin particles. Two intravenous injections or one intravenous and one intraperitoneal inoculation were much more effective. Experiments on route, interval, and dose suggested that the first dose of tin prepared the spleen in some manner while the second dose actually elicited the reaction. Pertussis vaccine or glucan could substitute for one of the tin injections. This new model is of interest for the study of plasma cell hyperplasia and also for revealing the pathogenic potential of metallic tin.
两次静脉注射金属锡粉导致脾脏显著、自限性肿大，可达正常大小的五到六倍。除了针对外来颗粒的上皮样细胞肉芽肿外，脾脏还含有大量成浆细胞、浆细胞以及含有拉塞尔小体的浆细胞。未成熟浆细胞的增殖活性通过有丝分裂和用氚标记胸腺嘧啶核苷标记的细胞的高发生率得以体现。相比之下，肉芽肿没有有丝分裂或标记。单次静脉注射锡通常在脾脏中几乎不产生反应或无反应，尽管有大量锡颗粒。两次静脉注射或一次静脉注射加一次腹腔接种则更有效。关于途径、间隔和剂量的实验表明，第一剂锡以某种方式使脾脏致敏，而第二剂实际上引发了反应。百日咳疫苗或葡聚糖可以替代其中一次锡注射。这个新模型对于研究浆细胞增生以及揭示金属锡的致病潜力具有重要意义。
