Windle J J, Shin H S, Morrow J F
J Immunol. 1984 Mar;132(3):1317-22.
Macrophages and P388D1 murine cultured cells produce interleukin 1 (lymphocyte-activating factor) when stimulated with lipopolysaccharide or other agents. Poly A+ RNA extracted from either type of stimulated cells and injected into Xenopus oocytes causes synthesis of material with the biologic and biochemical properties of interleukin 1. It potentiates lectin-mediated thymocyte proliferation, and it has the molecular dimensions of interleukin 1, as determined by gel exclusion chromatography. RNA from either cell type causes the synthesis of interleukin 1 with an isoelectric point of 4.8 to 5.0. RNA prepared from unstimulated macrophages or P388D1 cells does not cause interleukin 1 production by oocytes. We conclude that the amount of interleukin 1 mRNA increases greatly after stimulation of either cell type, and oocytes carry out any modifications of the polypeptide necessary for activity. The kinetics of interleukin 1 mRNA accumulation and of interleukin 1 production by macrophages and P388D1 cells are compared.
巨噬细胞和P388D1鼠培养细胞在受到脂多糖或其他试剂刺激时会产生白细胞介素1（淋巴细胞激活因子）。从任何一种受刺激细胞中提取的聚腺苷酸RNA（Poly A+ RNA）注射到非洲爪蟾卵母细胞中，都会促使具有白细胞介素1生物学和生化特性的物质合成。它能增强凝集素介导的胸腺细胞增殖，并且通过凝胶过滤色谱法测定，其分子大小与白细胞介素1相符。来自这两种细胞类型的RNA都会促使合成等电点为4.8至5.0的白细胞介素1。从未受刺激的巨噬细胞或P388D1细胞制备的RNA不会导致卵母细胞产生白细胞介素1。我们得出结论，在刺激任何一种细胞类型后，白细胞介素1信使核糖核酸（mRNA）的量会大幅增加，并且卵母细胞会对该多肽进行活性所需的任何修饰。比较了巨噬细胞和P388D1细胞中白细胞介素1 mRNA积累的动力学以及白细胞介素1产生的动力学。
