Stephens R, Traul K, Gaudreau P, Yeh J, Fisher L, Mayyasi S A
Int J Cancer. 1977 Mar 15;19(3):305-16. doi: 10.1002/ijc.2910190305.
Three groups of EBV antigens, VCA, MA and EA, were compared by the techniques of electron microscopic immunoperoxidase (IP) and immunofluorescence (IF). P3HR-1 and EBV superinfected Raji cells served as targets for labelled sera from patients with BL, NPC and IM or from healthy donors. 125I peroxidase-labelled antibodies were also prepared to determine, autoradiographically, the penetration of the complex into the cell system, and to monitor the incubation and washing procedures. The development of a gentle sedimentation technique proved critical in handling the fragile target cells. VCA and MA were readily identified and localized by both procedures without significant modification of the basic techniques. Indentification of early antigens by IP required modification of the fixation method to include a brief treatment in acetone. The diffuse early antigen (EAD) was found to be associated with cellular ribosomes.
通过电子显微镜免疫过氧化物酶（IP）和免疫荧光（IF）技术，对三组EBV抗原，即病毒衣壳抗原（VCA）、膜抗原（MA）和早期抗原（EA）进行了比较。P3HR - 1细胞和EBV超感染的Raji细胞作为来自伯基特淋巴瘤（BL）、鼻咽癌（NPC）和传染性单核细胞增多症（IM）患者或健康供体的标记血清的靶细胞。还制备了125I过氧化物酶标记抗体，用于通过放射自显影法确定复合物向细胞系统中的渗透情况，并监测孵育和洗涤过程。事实证明，一种温和的沉降技术对于处理脆弱的靶细胞至关重要。通过这两种方法都能很容易地识别和定位VCA和MA，且基本技术无需进行重大修改。通过IP鉴定早期抗原需要对固定方法进行修改，包括在丙酮中进行短暂处理。发现弥漫性早期抗原（EAD）与细胞核糖体有关。
