Raymond F L, Caton A J, Cox N J, Kendal A P, Brownlee G G
Nucleic Acids Res. 1983 Oct 25;11(20):7191-203. doi: 10.1093/nar/11.20.7191.
The sequence of the HA1 subunit region of the haemagglutinin gene of influenza A/USSR/90/77, and A/Brazil/11/78, A/Lackland/3/78, A/England/333/80 and A/India/6263/80 was determined by dideoxy-sequencing methods using total virion RNA and specific oligonucleotide primers for reverse transcriptase. These 1977-1980 strains share a minimum of 85% amino acid sequence homology with influenza A/PR/8/34. Most of the surface amino acid substitutions which occurred during the evolution of A/PR/8/34 to A/USSR/90/77 and subsequently in the 1978-1980 strains are located in the 4 antigenic sites previously defined by an analysis of laboratory-selected mutants of A/PR/8/34. We deduce an evolutionary pathway for the 1977-80 strains and suggest their different epidemic properties may be a consequence of only a few amino acid changes.
采用双脱氧测序法,使用总病毒粒子RNA和逆转录酶特异性寡核苷酸引物,测定了甲型流感病毒/苏联/90/77、甲型流感病毒/巴西/11/78、甲型流感病毒/拉克伦德/3/78、甲型流感病毒/英格兰/333/80和甲型流感病毒/印度/6263/80血凝素基因HA1亚基区域的序列。这些1977 - 1980年的毒株与甲型流感病毒/PR/8/34至少有85%的氨基酸序列同源性。在从甲型流感病毒/PR/8/34进化到甲型流感病毒/苏联/90/77以及随后在1978 - 1980年毒株进化过程中发生的大多数表面氨基酸替换,都位于先前通过对甲型流感病毒/PR/8/34实验室选择突变体分析所确定的4个抗原位点。我们推断了1977 - 80年毒株的进化途径,并表明它们不同的流行特性可能是仅少数氨基酸变化的结果。