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马唾液腺中糖缀合物的凝集素组织化学

Lectin histochemistry of glycoconjugates in horse salivary glands.

作者信息

Gargiulo A M, Pedini V, Ceccarelli P

机构信息

Istituto di Anatomia Normale degli Animali Domestici, Università degli Studi di Perugia, Italy.

出版信息

Anat Histol Embryol. 1993 Mar;22(1):83-90. doi: 10.1111/j.1439-0264.1993.tb00344.x.

DOI:10.1111/j.1439-0264.1993.tb00344.x
PMID:8489050
Abstract

The glycoconjugate content of major horse salivary glands was investigated by means of horseradish peroxidase-conjugated lectins. Qualitative differences were observed in the terminal sugar residues of secretory glycoproteins and glycoconjugates linked to the apical surface of excretory duct epithelial cells. Mucous acinar cells in mandibular and sublingual glands contained oligosaccharides with D-galactose, alpha- and beta-N-acetylgalactosamine, N-acetylglucosamine and fucose residues, whereas mandibular, sublingual and parotid serous cells contained only oligosaccharides with terminal alpha- and beta-N-acetylgalactosamine residues. The apical portion of striated and interlobular duct lining cells of mandibular and sublingual glands stained for alpha- and beta-N-acetylgalactosamine and for N-acetylglucosamine. In parotid gland the cytoplasm of intercalated duct cells and the apical surface of striated duct epithelial cells stained for alpha-N-acetylgalactosamine.

摘要

利用辣根过氧化物酶偶联凝集素对马主要唾液腺的糖缀合物含量进行了研究。在分泌性糖蛋白和与排泄管上皮细胞顶端表面相连的糖缀合物的末端糖残基中观察到了定性差异。下颌下腺和舌下腺的黏液腺泡细胞含有带有D-半乳糖、α-和β-N-乙酰半乳糖胺、N-乙酰葡糖胺和岩藻糖残基的寡糖,而下颌下腺、舌下腺和腮腺的浆液细胞仅含有带有末端α-和β-N-乙酰半乳糖胺残基的寡糖。下颌下腺和舌下腺的纹状管和小叶间管衬里细胞的顶端部分对α-和β-N-乙酰半乳糖胺以及N-乙酰葡糖胺呈阳性染色。在腮腺中,闰管细胞的细胞质和纹状管上皮细胞的顶端表面对α-N-乙酰半乳糖胺呈阳性染色。

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