使用单克隆亚类特异性抗体对IgG亚类进行印迹免疫固定。

Imprint immunofixation of IgG subclasses, using monoclonal subclass-specific antibodies.

作者信息

Vartdal F, Bird P

出版信息

Scand J Immunol. 1983 Oct;18(4):367-70. doi: 10.1111/j.1365-3083.1983.tb01809.x.

DOI:10.1111/j.1365-3083.1983.tb01809.x

Abstract

Monoclonal mouse hybridoma antibodies to human IgG subclasses 1-4 were used in an imprint immunofixation (IIF) technique to characterize the IgG subclass of a panel of IgG myeloma and normal sera separated by thin-layer electrofocusing. The results show that the antisera were IgG subclass-specific by this technique and that IIF can be a useful method in identifying IgG subclasses of IgG myeloma proteins in whole sera.

摘要

使用针对人IgG亚类1 - 4的单克隆小鼠杂交瘤抗体，通过印迹免疫固定（IIF）技术来鉴定一组经薄层电聚焦分离的IgG骨髓瘤和正常血清中IgG亚类的特征。结果表明，通过该技术抗血清具有IgG亚类特异性，并且IIF可作为鉴定全血清中IgG骨髓瘤蛋白IgG亚类的一种有用方法。