Person J M, Quintin-Colonna F, Barrat F, Le Garrec Y, Pilet C
Comp Immunol Microbiol Infect Dis. 1983;6(4):345-56. doi: 10.1016/0147-9571(83)90028-0.
A micromethod technique was used to evaluate in vitro sensitivity of the peripheric bovine lymphocytes obtained from a newly born calf, up to 3 months of age to different non-specific mitogens: Phytohemagglutinin (PHA) Concanavaline A (Con A) and Pokeweed Mitogen (PWM). The results obtained show that the calf lymphocytes respond to the 3 mitogens by a considerable cellular proliferation. The blastogenic response was found at various levels during the first 3 months of life, and appeared to stabilize at levels similar to the adult bovine. Highly sensitive variations were noted in the lymphocyte reactivity, notably with PHA and Con A. These results seem to indicate the existence of periods of T cell immunodeficiency, not only during the first few days after birth, but throughout the first months of the calves' life. It may also be indicative of the interest of immunostimulant therapy during this period, which needs further investigation.
采用微量方法技术评估了从新生犊牛获取的外周血淋巴细胞在3个月龄以内对不同非特异性有丝分裂原：植物血凝素（PHA）、刀豆蛋白A（Con A）和商陆有丝分裂原（PWM）的体外敏感性。所得结果表明，犊牛淋巴细胞对这3种有丝分裂原均有显著的细胞增殖反应。在出生后的前3个月内，增殖反应处于不同水平，且似乎稳定在与成年牛相似的水平。淋巴细胞反应性存在高度敏感的变化，尤其是对PHA和Con A。这些结果似乎表明，不仅在出生后的头几天，而且在犊牛生命的最初几个月中，都存在T细胞免疫缺陷期。这也可能表明在此期间免疫刺激疗法具有应用价值，这需要进一步研究。
