Kuperman O J, Bach F H
Scand J Immunol. 1977;6(3):161-72. doi: 10.1111/j.1365-3083.1977.tb00381.x.
The present experiments were undertaken to delineate the role of LD and SD antigens in terms of memory in cell-mediated immune response (CML). Using recombinants that differ for either the H-2K or H-2D determinants (SD-different) and other recombinants differing for the central region of H-2 (LD-different), we have investigated this question. The results indicate that priming to SD determinants leads to a more rapid and higher response when cells are restimulated in vitro with either the same SD antigen alone or the same SD plus an LD antigen and tested on target cells that possess either the SD antigen alone or both the LD and SD antigen. Antigens in the central regions of H-2, which includes LD, serve as the stimulus both for the helper T cells and for the cytotoxic T lymphocytes. Priming with a high dose of LD antigens leads to memory in the cytotoxic T lymphocyte recognizing the cytotoxic target antigens coded by the central region of H-2. Nevertheless, memory could not be detected when priming was done under the same conditions as for SD antigens. Moreover, a primed helper T-cell response does not facilitate the development of increased CML on restimulation in vitro with the LD antigen used for priming and a new SD antigen. Thus, it seems that memory in CML is in the cytotoxic T lymphocyte and not in the proliferating helper cells.
进行本实验是为了阐明LD和SD抗原在细胞介导的免疫反应（CML）记忆方面的作用。我们使用了在H-2K或H-2D决定簇上不同的重组体（SD不同）以及在H-2中央区域不同的其他重组体（LD不同）来研究这个问题。结果表明，当细胞在体外单独用相同的SD抗原或相同的SD加LD抗原重新刺激，并在仅拥有SD抗原或同时拥有LD和SD抗原的靶细胞上进行测试时，对SD决定簇的致敏会导致更快、更高的反应。H-2中央区域的抗原，包括LD，对辅助性T细胞和细胞毒性T淋巴细胞均起刺激作用。用高剂量的LD抗原进行致敏会导致细胞毒性T淋巴细胞产生记忆，该细胞毒性T淋巴细胞可识别由H-2中央区域编码的细胞毒性靶抗原。然而，在与SD抗原相同的条件下进行致敏时，无法检测到记忆。此外，致敏的辅助性T细胞反应在用用于致敏的LD抗原和新的SD抗原进行体外重新刺激时，并不会促进CML增加的发展。因此，似乎CML中的记忆存在于细胞毒性T淋巴细胞中，而非增殖的辅助性细胞中。
