Lopez-Berestein G, Reuben J, Hersh E M, Kilbourn R, Hester J P, Bielski M, Talpaz M, Mavligit G M
Transfusion. 1983 May-Jun;23(3):201-6. doi: 10.1046/j.1537-2995.1983.23383224895.x.
Large numbers (2.9 +/- 1.2 X 10(9)) of mononuclear cells can be obtained from incidental samples collected during routine plateletapheresis. We conducted studies comparing characteristics and functions of mononuclear cells derived from venous blood samples and from routine plateletapheresis in the same normal donors. Cell viability was similar in both samples (96 +/- 1% plateletapheresis vs 97 +/- 2% venous blood). Higher concentration of monocytes were observed in the plateletapheresis samples (32.3 +/- 6%) than in the venous blood (14.3 +/- 4%). The procedure of plateletapheresis does not seem to alter lymphocyte or monocyte function. Thus, the functional integrity of these cell populations was demonstrated in terms of natural killer cell activity, blastogenic response to mitogens, local graft-versus-host reactions, monocyte-mediated antibody-dependent cellular cytotoxicity against human red cells, monocyte-mediated tumor cell cytotoxicity, latex phagocytosis, and monocyte-dependent lymphocyte blastogenesis. We conclude that monocytes and lymphocytes obtained during routine plateletapheresis are functionally intact.
从常规血小板单采过程中收集的偶然样本中可获得大量（2.9±1.2×10⁹）单核细胞。我们进行了研究，比较了来自相同正常供体静脉血样本和常规血小板单采的单核细胞的特征和功能。两个样本中的细胞活力相似（血小板单采样本为96±1%，静脉血样本为97±2%）。血小板单采样本中观察到的单核细胞浓度（32.3±6%）高于静脉血（14.3±4%）。血小板单采过程似乎不会改变淋巴细胞或单核细胞的功能。因此，这些细胞群体的功能完整性在自然杀伤细胞活性、对有丝分裂原的增殖反应、局部移植物抗宿主反应、单核细胞介导的针对人红细胞的抗体依赖性细胞毒性、单核细胞介导的肿瘤细胞毒性、乳胶吞噬作用以及单核细胞依赖性淋巴细胞增殖方面得到了证实。我们得出结论，常规血小板单采过程中获得的单核细胞和淋巴细胞功能完整。
