Mintz P D, Normansell D E, Innes D J
J Clin Apher. 1987;3(3):164-6. doi: 10.1002/jca.2920030307.
To determine whether any lymphocyte subset is preferentially harvested and transfused as a consequence of plateletapheresis with the Fenwal CS-3000 Blood Cell Separator, the proportions of lymphocyte subpopulations in platelet concentrates were compared to their proportions in the donors' peripheral venous blood immediately prior to platelet collection. There was no difference in the proportion of B cells (surface immunoglobulin positive), T cells (OKT3 positive), helper/inducer T cells (OKT4 positive), suppressor/cytotoxic T cells (OKT8 positive), and natural killer cells (Leu 7 positive) in the donors' peripheral venous blood and the plateletapheresis product. Thus, although previous studies have demonstrated the ability to separate lymphocyte subpopulations by density centrifugation and velocity sedimentation, plateletapheresis with the CS-3000 harvests the lymphocyte subpopulations studied in the same proportions in which they circulate in donors' peripheral venous blood.
为了确定使用芬华CS - 3000血细胞分离机进行血小板单采时，是否会优先采集并输注任何淋巴细胞亚群，将血小板浓缩物中淋巴细胞亚群的比例与其采集血小板前供体外周静脉血中的比例进行了比较。供体外周静脉血和血小板单采产物中B细胞（表面免疫球蛋白阳性）、T细胞（OKT3阳性）、辅助/诱导性T细胞（OKT4阳性）、抑制/细胞毒性T细胞（OKT8阳性）和自然杀伤细胞（Leu 7阳性）的比例没有差异。因此，尽管先前的研究已经证明能够通过密度离心和速度沉降分离淋巴细胞亚群，但使用CS - 3000进行血小板单采时，所研究的淋巴细胞亚群的采集比例与它们在供体外周静脉血中循环的比例相同。
