3,5-二碘甲状腺原氨酸的放射免疫测定法。

A radioimmunoassay for 3,5-diiodothyronine.

作者信息

Meinhold H, Schürnbrand P

出版信息

Clin Endocrinol (Oxf). 1978 Jun;8(6):493-7. doi: 10.1111/j.1365-2265.1978.tb02187.x.

DOI:10.1111/j.1365-2265.1978.tb02187.x

Abstract

A radioimmunoassay for the measurement of 3,5-diiodothyronine (3,5-T2) using a 3H-labelled derivative of 3,5-T2 as tracer has been developed. The cross-reactivity of the antiserum was relatively high for T3 (3.1%) and low for T4 (less than 0.0015%). 3,5-T2 serum concentrations measured after fractional enrichment of 3,5-T2 were near or below the detection limit of 0.1 nmol/l in ten normal subjects and 0.38 nmol/l in five hyperthyroid patients. 3,5-T2 was also detected in thyroid tissue. The serum half-time of 3,5-T2 estimated after injection of 2 mg 3,5-T2 in three normal volunteers was 4.5 h.

摘要

已开发出一种放射免疫分析法，用于测定3,5 - 二碘甲状腺原氨酸（3,5 - T2），该方法使用3,5 - T2的3H标记衍生物作为示踪剂。抗血清对T3的交叉反应率相对较高（3.1%），对T4的交叉反应率较低（低于0.0015%）。在十名正常受试者中，对3,5 - T2进行分级富集后测得的3,5 - T2血清浓度接近或低于检测限0.1 nmol/l，在五名甲状腺功能亢进患者中为0.38 nmol/l。在甲状腺组织中也检测到了3,5 - T2。在三名正常志愿者中注射2 mg 3,5 - T2后估计的3,5 - T2血清半衰期为4.5小时。