Lenfant M, Millerioux L, Blazsek I, Duchange N
Immunology. 1983 Apr;48(4):635-45.
A spleen-derived immunosuppressive peptide (SDIP) has been purified to homogeneity. Its physicochemical properties (electrophoretic mobility, u.v. spectra, absence of dansyl derivative) and its enzymatic susceptibilities (proteolytic enzymes, RNase, and DNase) were similar to those of the thymic hormone 'FTS'. SDIP and FTS were eluted with identical retention times in high performance liquid chromatography analysis in three different systems. When tested in sheep cell rosettes, and in the FTS radioimmunoassay in J.F. Bach's laboratory, SDIP presented an activity similar to FTS. In order to compare the thymic hormone to SDIP the biological activity of FTS was determined in in vivo and in in vitro humoral immunity reactions to a T-dependent antigen. As SDIP, FTS inhibited in vivo and in vitro the 19S-bearing cell formation during the last step of the differentiation of the lymphocytes, in the same range of concentration. The two factors appeared to stimulate the incorporation of [3H]-thymidine into the DNA of short-term cultures of thymocytes. The similarity of biological properties of SDIP and FTS together with the similarity observed in the physico-chemical and biochemical properties led to the conclusion that bovine spleen contains a factor similar to FTS.
一种源自脾脏的免疫抑制肽（SDIP）已被纯化至同质。其物理化学性质（电泳迁移率、紫外光谱、无丹磺酰衍生物）及其对酶的敏感性（蛋白水解酶、核糖核酸酶和脱氧核糖核酸酶）与胸腺激素“FTS”相似。在三种不同系统的高效液相色谱分析中，SDIP和FTS以相同的保留时间被洗脱。当在绵羊细胞玫瑰花结试验以及在J.F.巴赫实验室的FTS放射免疫分析中进行测试时，SDIP表现出与FTS相似的活性。为了将胸腺激素与SDIP进行比较，在对T依赖性抗原的体内和体外体液免疫反应中测定了FTS的生物活性。与SDIP一样，FTS在相同浓度范围内，在淋巴细胞分化的最后阶段抑制含19S细胞的形成。这两种因子似乎都能刺激[3H] - 胸腺嘧啶核苷掺入胸腺细胞短期培养物的DNA中。SDIP和FTS生物学特性的相似性，以及在物理化学和生化特性方面观察到的相似性，得出牛脾脏含有一种与FTS相似的因子的结论。
