Michael-Marler S, Brown M L, Siebeling R J
Appl Environ Microbiol. 1983 Mar;45(3):748-54. doi: 10.1128/aem.45.3.748-754.1983.
Turtle eggs, 24 h old, were infected with Arizona hinshawii and treated 48 h later with gentamicin sulfate (Garasol; Shering Corp., Allantown, N.J.) by pressure differential egg dip treatment to ascertain the concentration of this reagent required to eradicate this pathogen from eggs. Infected eggs treated with 1,000 or 1,500 micrograms of gentamicin per ml of dip solution eliminated detectable A. hinshawii from eggs as determined by testing shells and embryo-yolk homogenates of 12-day-old eggs and the gastrointestinal tracts, kidneys, livers and gall bladders, and yolks of 50-day-old embryos. Treated eggs produced hatchlings which did not excrete detectable A. hinshawii at 72 h or 30 days after hatching, nor was this organism recovered from the visceral organs of these hatchlings when necropsied 30 days after hatching. Bacteriological assays on infected nontreated eggs showed that greater than 70% of the eggs harbored A. hinshawii, and eggs in this group produced hatchlings which actively excreted and harbored A. hinshawii. Eggs not infected or treated also produced turtles which excreted and systemically carried A. hinshawii and Salmonella spp. though not at the same level as did the turtles produced from infected, nontreated eggs.
将24小时龄的龟蛋感染欣肖亚利桑那菌，48小时后通过压差浸蛋处理用硫酸庆大霉素（加雷索尔；先灵公司，新泽西州阿伦敦）进行处理，以确定从蛋中根除这种病原体所需的该试剂浓度。用每毫升浸液含1000或1500微克庆大霉素处理的感染蛋，通过检测12日龄蛋的蛋壳和胚胎 - 卵黄匀浆以及50日龄胚胎的胃肠道、肾脏、肝脏、胆囊和卵黄，确定从蛋中消除了可检测到的欣肖亚利桑那菌。经处理的蛋孵出的幼龟在孵化后72小时或30天未排出可检测到的欣肖亚利桑那菌，在孵化后30天进行尸检时，也未从这些幼龟的内脏器官中分离出该菌。对未处理的感染蛋进行的细菌学检测表明，超过70%的蛋携带欣肖亚利桑那菌，该组蛋孵出的幼龟会主动排出并携带欣肖亚利桑那菌。未感染或未处理的蛋也孵出了排出并全身携带欣肖亚利桑那菌和沙门氏菌属的龟，不过携带水平与未处理的感染蛋孵出的龟不同。
