Bowman A, Gillespie J S
J Physiol. 1983 Aug;341:603-16. doi: 10.1113/jphysiol.1983.sp014827.
Field stimulation of isolated, perfused bovine or canine penile arteries produced dilatation, after the adrenergic motor component of the response had been blocked with guanethidine and the vessels had developed a background tone. The vasodilatation was blocked by tetrodotoxin but not by atropine. The vasodilator responses to field stimulation were compared with those produced by ATP, by vasoactive intestinal peptide (VIP), and by the inhibitory factor extracted from the bovine retractor penis muscle. Of the three putative transmitters, the inhibitory factor produced responses that most closely resembled those to field stimulation. Haemoglobin, which blocks non-adrenergic, non-cholinergic inhibitory transmission in the bovine and canine retractor penis muscles, did not impair the vasodilatations produced by ATP or VIP, but slowly reduced or abolished those produced by field stimulation or by the inhibitory factor. Haemoglobin itself produced a powerful constriction of the isolated penile arteries. The results are compatable with the possibility that the inhibitory factor from the bovine retractor penis muscle (which may be the inhibitory transmitter in that muscle) is, or closely resembles, the transmitter of non-adrenergic, non-cholinergic vasodilator fibres in the penile arteries of dog and ox.
在用胍乙啶阻断反应的肾上腺素能运动成分且血管产生基础张力后，对分离的灌注牛或犬阴茎动脉进行场刺激可导致血管扩张。这种血管舒张被河豚毒素阻断，但不被阿托品阻断。将场刺激引起的血管舒张反应与ATP、血管活性肠肽（VIP）以及从牛阴茎退缩肌中提取的抑制因子所产生的反应进行了比较。在这三种假定的递质中，抑制因子产生的反应与场刺激产生的反应最为相似。血红蛋白可阻断牛和犬阴茎退缩肌中的非肾上腺素能、非胆碱能抑制性传递，它不会削弱ATP或VIP所引起的血管舒张，但会缓慢降低或消除场刺激或抑制因子所引起的血管舒张。血红蛋白本身可使分离的阴茎动脉强烈收缩。这些结果支持这样一种可能性，即来自牛阴茎退缩肌的抑制因子（可能是该肌肉中的抑制性递质）是或类似于犬和牛阴茎动脉中非肾上腺素能、非胆碱能血管舒张纤维的递质。
