Bowman A, Gillespie J S, Martin W
Br J Pharmacol. 1981 Feb;72(2):365-72. doi: 10.1111/j.1476-5381.1981.tb09136.x.
1 A partially purified material has been isolated from methanol extracts of the bovine retractor penis muscle. This material exerts biological activity only after treatment with acid and subsequent neutralisation. The active principle in this extract, which appears to be no known autacoid, mimics the response to stimulation of the non-adrenergic, non-cholinergic nerves in the bovine isolated retractor penis muscle. 2 This inhibitory extract did not alter the heart rate or blood pressure of the anaesthetized rat when administered either by intravenous or intra-arterial injection, nor did it have any obvious effect on isolated cardiac muscle. 4 The extract produced relaxation of spiral strips of various arteries isolated from ox, cat, rabbit or rat, in which tone was induced by noradrenaline, K+ of Ba2+. 5 The extract also produced dilatation of the resistance vessels of the rat isolated mesenteric circulation and the rat hindquarters perfused with Krebs solution; tone was induced in these vessels by adrenaline or noradrenaline. 6 Lack of vasodilator activity of the extract in the whole animal appeared to be due to rapid inactivation in the blood, probably by binding to the erythrocytes.
1 已从牛阴茎退缩肌的甲醇提取物中分离出一种部分纯化的物质。该物质仅在经过酸处理并随后中和后才发挥生物活性。这种提取物中的活性成分似乎不是已知的自分泌物质，它模拟了对牛离体阴茎退缩肌中去甲肾上腺素能、非胆碱能神经刺激的反应。2 当通过静脉或动脉内注射给予这种抑制性提取物时，它不会改变麻醉大鼠的心率或血压，对离体心肌也没有任何明显影响。4 该提取物能使从牛、猫、兔或大鼠分离出的各种动脉的螺旋条松弛，这些动脉条的张力是由去甲肾上腺素、K⁺或Ba²⁺诱导产生的。5 该提取物还能使大鼠离体肠系膜循环和用 Krebs 溶液灌注的大鼠后肢的阻力血管扩张；这些血管的张力是由肾上腺素或去甲肾上腺素诱导产生的。6 提取物在整个动物体内缺乏血管舒张活性似乎是由于在血液中迅速失活，可能是通过与红细胞结合。
