Flügel-Link R M, Jones M R, Kopple J D
JPEN J Parenter Enteral Nutr. 1983 Sep-Oct;7(5):450-6. doi: 10.1177/0148607183007005450.
Red cell and plasma amino acid concentrations were measured in nine healthy subjects, nine patients with advanced chronic renal failure, and before and after dialysis in eight patients undergoing maintenance hemodialysis. Blood was obtained after an overnight fast except for postdialysis specimens. In red cells from the chronically uremic patients and in predialysis specimens, there was increased histidine, cystine, glutamic acid, glycine, ornithine, citrulline, taurine, and N tau-methylhistidine, and an increased glycine to serine ratio. Red cell valine, tyrosine, and the ratios of tyrosine to phenylalanine and valine to glycine were decreased in the two groups. Many amino acid levels that were abnormal in red cells were also abnormal in plasma. However, several amino acids were altered in only one of these compartments. Some red cell or plasma amino acid concentrations were abnormal in the uremic or hemodialysis patients but not in both. During hemodialysis, red cell amino acids did not decrease as markedly as did the plasma concentrations. These findings indicate that in chronically uremic and hemodialysis patients there is an abnormal amino acid pattern in both red cells and plasma. Although the altered amino acid patterns in these two compartments have similarities, they are not identical.
测定了9名健康受试者、9名晚期慢性肾衰竭患者以及8名接受维持性血液透析患者透析前后的红细胞和血浆氨基酸浓度。除透析后标本外，均在空腹过夜后采血。在慢性尿毒症患者的红细胞和透析前标本中，组氨酸、胱氨酸、谷氨酸、甘氨酸、鸟氨酸、瓜氨酸、牛磺酸和N-τ-甲基组氨酸增加，甘氨酸与丝氨酸的比例升高。两组患者红细胞中的缬氨酸、酪氨酸以及酪氨酸与苯丙氨酸的比例和缬氨酸与甘氨酸的比例均降低。红细胞中许多异常的氨基酸水平在血浆中也异常。然而，有几种氨基酸仅在其中一个区室发生改变。尿毒症或血液透析患者的一些红细胞或血浆氨基酸浓度异常，但并非两者都异常。在血液透析过程中，红细胞氨基酸的降低幅度不如血浆浓度明显。这些发现表明，慢性尿毒症和血液透析患者的红细胞和血浆中氨基酸模式均异常。尽管这两个区室中氨基酸模式的改变有相似之处，但并不完全相同。
