Egan M L, Mendelsohn S L, Abo T, Balch C M
Arthritis Rheum. 1983 May;26(5):623-9. doi: 10.1002/art.1780260508.
A monoclonal antibody, HNK-1, that detects a differentiation antigen on human granular lymphocytes with natural killer (NK) activity was used to enumerate this subpopulation in the peripheral blood of 14 patients with systemic lupus erythematosus (SLE). Nine patients had severely decreased numbers of HNK-1+ cells, 3 patients had elevated levels of HNK-1+ cells, and 2 patients had appropriate numbers of HNK-1+ cells compared with the levels in 112 normal controls. All SLE patients exhibited low NK killing ability against K562 target cells compared with controls. An increased proportion of the HNK-1+ cells was categorized as immature granular lymphocytes in over 50% of the SLE patients because their HNK-1+ cells coexpressed the OKT3 antigen and contained a paucity of cytoplasmic granules. The numbers of HNK-1+ cells or the HNK-1+ OKT3+ subgroup did not correlate with steroid therapy. This evidence suggests that levels of HNK-1+ lymphocytes are abnormal and functionally immature in most SLE patients. Longitudinal studies conducted over several months on a number of SLE patients demonstrated fluctuations in the ratio of mature and immature HNK-1+ cells and total HNK-1+ cells. Additional patients tested over longer periods of time will have to be studied to determine whether the proportion of mature NK cells (HNK-1+ OKT-) and immature NK cells (HNK-1+ OKT3+) will be useful in predicting the clinical course of disease.
一种单克隆抗体HNK - 1可检测具有自然杀伤（NK）活性的人颗粒淋巴细胞上的分化抗原，用其对14例系统性红斑狼疮（SLE）患者外周血中的这一亚群进行计数。与112名正常对照者相比，9例患者的HNK - 1+细胞数量严重减少，3例患者的HNK - 1+细胞水平升高，2例患者的HNK - 1+细胞数量正常。与对照组相比，所有SLE患者对K562靶细胞的NK杀伤能力均较低。在超过50%的SLE患者中，HNK - 1+细胞比例增加的部分被归类为未成熟颗粒淋巴细胞，因为其HNK - 1+细胞共表达OKT3抗原且胞质颗粒较少。HNK - 1+细胞或HNK - 1+ OKT3+亚组的数量与类固醇治疗无关。这一证据表明，大多数SLE患者中HNK - 1+淋巴细胞水平异常且功能不成熟。对一些SLE患者进行的长达数月的纵向研究显示，成熟和未成熟HNK - 1+细胞以及总HNK - 1+细胞的比例存在波动。还需要对更长时间内检测的更多患者进行研究，以确定成熟NK细胞（HNK - 1+ OKT -）和未成熟NK细胞（HNK - 1+ OKT3+）的比例是否有助于预测疾病的临床进程。
