van Asbeck B S, Marx J J, Struyvenberg A, van Kats J H, Verhoef J
J Immunol. 1984 Feb;132(2):851-6.
FeCl3 or Fe(III) that attached to chelating ligands such as citrate or nitrilotriacetic acid (NTA) at a molar ratio of 1:1 had a toxic effect on PMN. Uptake of radiolabeled Staphylococcus aureus by PMN, preincubated for 2 hr at 37 degrees C in a medium containing Fe(III)-citrate or Fe(III)-NTA, was significantly lower than that of control PMN preincubated without excess iron (p less than 0.002). However, at a 1:2 molar ratio of Fe(III) to citrate or NTA, the iron was not toxic. In contrast, the iron-liganding molecules transferrin and deferoxamine protected the PMN against the noxious effect of iron at concentrations just high enough to sequester all the iron. Fe(III) increased the generation of luminol chemiluminescence by stimulated PMN, whereas the oxygen consumption of the cells was not altered in the presence of Fe(III); this suggests a catalytic effect of iron on the production by PMN of oxygen metabolites at some step beyond the formation of superoxide. No effect of iron was observed when the incubation was performed at 4 degrees C, nor when an oxygen-radical scavenger such as thiourea, mannitol, or catalase was present in the incubation medium. Also, Fe(III) had much less effect on the phagocytic function of PMN of a patient with chronic granulomatous disease. The results indicate that the Fe(III)-induced defect in the phagocytic capacity of PMN depends on the nature and the concentration of the ligand attached to the iron ion, and also suggest that the noxious effect of iron on the PMN function is a result of its ability to catalyze the generation of toxic oxygen species by these cells.
以1:1摩尔比与螯合配体（如柠檬酸盐或次氮基三乙酸（NTA））结合的FeCl3或Fe(III)对中性粒细胞具有毒性作用。在含有Fe(III)-柠檬酸盐或Fe(III)-NTA的培养基中于37℃预孵育2小时的中性粒细胞对放射性标记金黄色葡萄球菌的摄取，显著低于未用过量铁预孵育的对照中性粒细胞（p<0.002）。然而，当Fe(III)与柠檬酸盐或NTA的摩尔比为1:2时，铁没有毒性。相反，铁配体分子转铁蛋白和去铁胺在浓度刚好足以螯合所有铁的情况下，保护中性粒细胞免受铁的有害影响。Fe(III)增加了受刺激的中性粒细胞产生鲁米诺化学发光，而在Fe(III)存在的情况下细胞的耗氧量未改变；这表明铁在超氧化物形成之外的某个步骤对中性粒细胞产生氧代谢产物具有催化作用。当在4℃进行孵育时，未观察到铁的影响，当孵育培养基中存在氧自由基清除剂如硫脲、甘露醇或过氧化氢酶时也未观察到铁的影响。此外，Fe(III)对慢性肉芽肿病患者中性粒细胞的吞噬功能影响小得多。结果表明，Fe(III)诱导的中性粒细胞吞噬能力缺陷取决于与铁离子结合的配体的性质和浓度，并且还表明铁对中性粒细胞功能的有害作用是其催化这些细胞产生有毒氧物种能力的结果。
