Hirono I, Yamada K, Niwa H, Shizuri Y, Ojika M, Hosaka S, Yamaji T, Wakamatsu K, Kigoshi H, Niiyama K
Cancer Lett. 1984 Jan;21(3):239-46. doi: 10.1016/0304-3835(84)90001-6.
Isolation of the carcinogen in the boiling water extract of bracken fern was conducted by following the active principle with a carcinogenicity bioassay. Fractionation of the bracken extract was carried out using adsorption on resin (Amberlite XAD-2 and TOYOPEARL HW-40 (c] and organic solvent extraction. A diet containing each of the fractions was given to 7 female Charles River Sprague-Dawley rats (CD rats) of 4 weeks old, except for the second fraction. All 7 rats given the last carcinogenic fraction developed mammary and intestinal tumors and 5 rats had urinary bladder tumors. Ptaquiloside (PT) which induced mammary cancer in female CD rats and rho-hydroxystyrene glycosides were isolated from this fraction.
通过致癌性生物测定法追踪活性成分，对蕨菜沸水提取物中的致癌物进行分离。蕨菜提取物的分级分离采用树脂吸附法（Amberlite XAD - 2和TOYOPEARL HW - 40（c））和有机溶剂萃取法。除了第二级分之外，将含有各级分的饲料喂给7只4周龄的雌性查尔斯河斯普拉格 - 道利大鼠（CD大鼠）。给予最后一级致癌级分的所有7只大鼠均出现乳腺和肠道肿瘤，5只大鼠出现膀胱肿瘤。从该级分中分离出在雌性CD大鼠中诱发乳腺癌的ptaquiloside（PT）和对羟基苯乙烯糖苷。
