Quirico Santos T, Valdimarsson H
Cell Immunol. 1984 Feb;83(2):442-6. doi: 10.1016/0008-8749(84)90324-1.
Introduction of T-dependent antigens into the subarachnoid space (isas) resulted in higher systemic antibody responses in mice than injections into the peritoneal cavity (ip) or other sites commonly used for immunization. Antibody production in isas immunized mice was not increased by treatment with cyclophosphamide (Cy) at doses known to abolish T-suppressor-cell activity, but such treatment increased antibody production in ip immunized mice toward the higher level which was observed in the isas immunized animals. Suppressor cell-dependent low zone tolerance (LZT) to TNP-BSA could not be induced by isas injections of deaggregated BSA (d-BSA). Conversely, mice which were unresponsive to ip injected d-BSA showed consistent systemic antibody responses when the antigen was injected isas. These observations indicate that immune responses initiated within the CNS are associated with relatively ineffective induction of systemic suppressor cell activity.
将T依赖性抗原引入蛛网膜下腔（isas），相比于注入腹腔（ip）或其他常用免疫部位，能使小鼠产生更高的全身抗体反应。已知剂量的环磷酰胺（Cy）可消除T抑制细胞活性，用其处理isas免疫的小鼠，抗体产生并未增加，但这种处理使ip免疫的小鼠抗体产生增加至isas免疫动物所观察到的较高水平。isas注射去聚合牛血清白蛋白（d-BSA）不能诱导对TNP-BSA的抑制细胞依赖性低区耐受性（LZT）。相反，对ip注射d-BSA无反应的小鼠，当抗原经isas注射时，会表现出一致的全身抗体反应。这些观察结果表明，中枢神经系统内引发的免疫反应与全身抑制细胞活性的诱导相对无效有关。
