Newman M A, Que Hee S S
Clin Chem. 1984 Feb;30(2):308-10.
We investigated three methods for determination of cholinesterase (ChE) in human blood sera, using reference sera, sera of known ChE content, and serum variously diluted with deactivated sera. The methods were the Du Pont aca analyzer, the Pfizer "ChE-tel" method, and the Michel method with and without direct reagent-blank correction. With respect to precision and reproducibility the methods ranked: aca greater than Michel method corrected directly for reagent pH changes (Michel) greater than ChE-tel method greater than Michel method corrected indirectly for reagent pH changes. The interrelationships derived, in terms of each method's individual units were: Michel = (0.054 +/- 0.001) aca - (0.043 +/- 0.086), r2 = 0.947 ChE-tel = (4.44 +/- 0.30) aca - (5.11 +/- 0.99), r2 = 0.959 ChE-tel = (80.2 +/- 5.8) Michel + (0.028 +/- 0.240), r2 = 0.947 The ChE-tel method was subject to variations due to the quality of different kits. The aca results were validated by a collaborative laboratory study. Sera of known activity can be used effectively for accurate calibration.
我们使用参比血清、已知胆碱酯酶（ChE）含量的血清以及用灭活血清不同程度稀释的血清，研究了三种测定人血清中胆碱酯酶（ChE）的方法。这些方法分别是杜邦aca分析仪法、辉瑞“ChE-tel”法以及有无直接试剂空白校正的米歇尔法。在精密度和重现性方面，这些方法的排名为：aca法＞直接针对试剂pH变化校正的米歇尔法（米歇尔法）＞ChE-tel法＞间接针对试剂pH变化校正的米歇尔法。根据每种方法各自的单位得出的相互关系为：米歇尔法 = (0.054 ± 0.001)aca法 - (0.043 ± 0.086)，r2 = 0.947；ChE-tel法 = (4.44 ± 0.30)aca法 - (5.11 ± 0.99)，r2 = 0.959；ChE-tel法 = (80.2 ± 5.8)米歇尔法 + (0.028 ± 0.240)，r2 = 0.947。ChE-tel法会因不同试剂盒的质量而产生变化。aca法的结果通过一项协作实验室研究得到了验证。已知活性的血清可有效地用于准确校准。
