Rahi A H, Misra R N, Morgan G
Br J Ophthalmol. 1977 Mar;61(3):164-76. doi: 10.1136/bjo.61.3.164.
In experimental rabbits heterologous soluble lens proteins consisting of alpha-, beta-, and gamma-crystallins were found to be antigenic; they stimulated a marked antibody response compared to a rather weak T-cell response. The serum antibodies to alpha-crystallins appeared first, to be followed by antibodies to beta- and gamma-crystallins in that order. The rabbits did not respond to heterologous gamma-crystallins unless these were injected with Freund's adjuvant containing mycobacteria. Incomplete Freund's adjuvant (i.e., without mycobacteria) was found to be an inferior immunoaccelerator so far as lens antigens are concerned. The response to lenticular antigens in both magnitude and duration varied in different rabbits, which suggested to us the important role played by a central control mechanism involving the immune response (Ir) genes. Some of the antibodies in potent lens antisera cross-reacted with mitochondria, endoplasmic reticulum (i.e., microsomes), contractile organelles, and cell nuclei. This explains for the first time at least in part the reasons for the widely observed phenomenon of the reactivity of lens antisera with ocular and extraocular structures. Antibodies to soluble lens proteins as detected by immunofluorescence and immunoperoxidase techniques were shown to be of the IgG class. Systemic heterologous immunisation followed by discission of the lens does not lead to the typical changes of phakoallergic endophthalmitis in the rabbit.
在实验兔中，发现由α-、β-和γ-晶状体蛋白组成的异种可溶性晶状体蛋白具有抗原性；与相当微弱的T细胞反应相比，它们能刺激显著的抗体反应。针对α-晶状体蛋白的血清抗体首先出现，随后依次是针对β-和γ-晶状体蛋白的抗体。兔子对异种γ-晶状体蛋白无反应，除非将其与含分枝杆菌的弗氏佐剂一起注射。就晶状体抗原而言，不完全弗氏佐剂（即不含分枝杆菌）被发现是一种较差的免疫促进剂。不同兔子对晶状体抗原的反应在强度和持续时间上各不相同，这向我们表明涉及免疫反应（Ir）基因的中枢控制机制发挥了重要作用。高效价晶状体抗血清中的一些抗体与线粒体、内质网（即微粒体）、收缩性细胞器和细胞核发生交叉反应。这至少首次部分解释了晶状体抗血清与眼内和眼外结构发生反应这一广泛观察到的现象的原因。通过免疫荧光和免疫过氧化物酶技术检测到的针对可溶性晶状体蛋白的抗体显示为IgG类。全身性异种免疫后切除晶状体并不会导致兔子发生典型的晶状体过敏性眼内炎变化。
