Patel M, Shine B, Murray P I
Department of Pathology, Institute of Ophthalmology, London, United Kingdom.
Int Ophthalmol. 1990 Mar;14(2):97-100. doi: 10.1007/BF00154208.
A new technique measuring serum antoantibodies to lens proteins, employing antigen linked to magnetisable cellulose particles and fluorescent end-point detection, was used to examine patients with senile cataract and inflammatory eye disease. 40% of patients with senile cataract had antibodies to lens proteins, as did 28% of patients with heterochromic cyclitis and 20% of patients with uveitis, while there were no positive sera from patients with scleritis. The single patient with lens-induced uveitis had a high titre, but so did some patients with senile cataract. With the incorporation of a standard curve this technique offers a reliable quantitative assay for lens autoantibodies. It may provide a valuable tool for the investigation of pathogenetic mechanisms in cataract and inflammatory eye disease.
一种测量血清中晶状体蛋白自身抗体的新技术，该技术采用与可磁化纤维素颗粒相连的抗原及荧光终点检测法，用于检查老年性白内障患者和炎性眼病患者。40%的老年性白内障患者有晶状体蛋白抗体，28%的异色性睫状体炎患者及20%的葡萄膜炎患者也有该抗体，而巩膜炎患者的血清均为阴性。唯一一例晶状体诱发性葡萄膜炎患者抗体滴度很高，但一些老年性白内障患者的抗体滴度也很高。通过纳入标准曲线，该技术为晶状体自身抗体提供了一种可靠的定量检测方法。它可能为研究白内障和炎性眼病的发病机制提供一个有价值的工具。
