Weber L, Kirsch E, Müller P, Krieg T
J Invest Dermatol. 1984 Feb;82(2):156-60. doi: 10.1111/1523-1747.ep12259720.
Human skin is composed of several layers which are characterized by a specific macromolecular organization of connective tissue. Three approaches were used to quantify the collagen types present in each of the different layers: biochemical analysis of authentic tissue, metabolic labeling of organ cultures, and metabolic labeling of fibroblast monolayers. We obtained reproducible evidence for a somewhat higher ratio of type III/type I collagen synthesis in the papillary dermis and the subcutaneous fat compared to the reticular layer. Constant amounts of alpha 1 (I) trimers and type V collagen were found in all layers. The degree of hydroxylation of lysine in either type I or type III collagen was the same in any layer of the skin.
人类皮肤由几层组成，其特征是结缔组织具有特定的大分子结构。我们采用了三种方法来量化不同层中存在的胶原类型：对真实组织进行生化分析、对器官培养物进行代谢标记以及对成纤维细胞单层进行代谢标记。我们获得了可重复的证据，表明与网状层相比，乳头层真皮和皮下脂肪中III型/ I型胶原合成的比例略高。在所有层中均发现了恒定数量的α1（I）三聚体和V型胶原。在皮肤的任何一层中，I型或III型胶原中赖氨酸的羟基化程度均相同。
