Yen Chun-Ming, Shen Chiung-Chyi, Yang Yi-Chin, Liu Bai-Shuan, Lee Hsu-Tung, Sheu Meei-Ling, Tsai Meng-Hsiun, Cheng Wen-Yu
Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital; Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan, China.
Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital; Department of Physical Therapy, Hungkuang University; Basic Medical Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan, China.
Neural Regen Res. 2019 Sep;14(9):1617-1625. doi: 10.4103/1673-5374.255997.
Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury. Natural biopolymers have received much attention because of their biocompatibility. To investigate the effects of novel electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits (biopolymer nanofiber conduits) on the repair of peripheral nerve injury, we bridged 10-mm-long sciatic nerve defects with electrospun absorbable biopolymer nanofiber conduits, poly(ε-caprolactone) or silicone conduits in Sprague-Dawley rats. Rat neurologica1 function was weekly evaluated using sciatic function index within 8 weeks after repair. Eight weeks after repair, sciatic nerve myelin sheaths and axon morphology were observed by osmium tetroxide staining, hematoxylin-eosin staining, and transmission electron microscopy. S-100 (Schwann cell marker) and CD4 (inflammatory marker) immunoreactivities in sciatic nerve were detected by immunohistochemistry. In rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits, no serious inflammatory reactions were observed in rat hind limbs, the morphology of myelin sheaths in the injured sciatic nerve was close to normal. CD4 immunoreactivity was obviously weaker in rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits than in those subjected to repair with poly(ε-caprolactone) or silicone. Rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits tended to have greater sciatic nerve function recovery than those receiving poly(ε-caprolactone) or silicone repair. These results suggest that electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits have the potential of repairing sciatic nerve defects and exhibit good biocompatibility. All experimental procedures were approved by Institutional Animal Care and Use Committee of Taichung Veteran General Hospital, Taiwan, China (La-1031218) on October 2, 2014.
近期研究表明，人工合成导管在修复周围神经损伤方面具有潜力。天然生物聚合物因其生物相容性而备受关注。为研究新型电纺可吸收聚（ε-己内酯）/I型胶原纳米纤维导管（生物聚合物纳米纤维导管）对周围神经损伤修复的影响，我们在Sprague-Dawley大鼠中用该电纺可吸收生物聚合物纳米纤维导管、聚（ε-己内酯）或硅胶导管桥接10毫米长的坐骨神经缺损。在修复后8周内，每周使用坐骨神经功能指数评估大鼠的神经功能。修复8周后，通过四氧化锇染色、苏木精-伊红染色和透射电子显微镜观察坐骨神经的髓鞘和轴突形态。通过免疫组织化学检测坐骨神经中的S-100（施万细胞标志物）和CD4（炎症标志物）免疫反应性。在用该电纺可吸收生物聚合物纳米纤维导管修复的大鼠中，未观察到大鼠后肢出现严重炎症反应，受损坐骨神经的髓鞘形态接近正常。在用该电纺可吸收生物聚合物纳米纤维导管修复的大鼠中，CD4免疫反应性明显弱于用聚（ε-己内酯）或硅胶修复的大鼠。与接受聚（ε-己内酯）或硅胶修复的大鼠相比，用电纺可吸收生物聚合物纳米纤维导管修复的大鼠坐骨神经功能恢复往往更好。这些结果表明，电纺可吸收聚（ε-己内酯）/I型胶原纳米纤维导管具有修复坐骨神经缺损的潜力，并表现出良好的生物相容性。所有实验程序均于2014年10月2日获得中国台湾台中荣民总医院机构动物护理与使用委员会（La-1031218）的批准。
