Williams J M, Brandt K D
Arthritis Rheum. 1984 Feb;27(2):208-16. doi: 10.1002/art.1780270213.
We have previously shown that immobilization protects against the development of mechanically-induced osteoarthritis following-anterior cruciate ligament transection in dogs. Herein, we examine the effect of immobilization of the leg on the chemically-induced degeneration of femoral articular cartilage caused by intraarticular injection of iodoacetate in guinea pigs. One week after the injection, cartilage from animals which were not immobilized exhibited a decrease in Safranin O staining and a 10-20% reduction in the number of chondrocytes. Three weeks after injection, cell death and loss of Safranin O staining had progressed, and surface fibrillation and osteophytes had developed. Articular cartilage from the contralateral (uninjected) knees of guinea pigs which received iodoacetate, and from knees of animals which were immobilized for 1 week but did not receive iodoacetate, was histologically and histochemically normal. However, specimens from 2 of 4 untreated knees which were immobilized for 3 weeks showed a reduction in Safranin O staining. Immobilization of the knee did not alter the loss of Safranin O staining seen after intraarticular iodoacetate injection, but did reduce the depletion of chondrocytes (P less than 0.05). Furthermore, neither osteophytes nor fibrillation developed in any of the animals which were constrained after iodoacetate injection. Thus, immobilization was clearly protective in this model of chemically-induced cartilage injury.
我们之前已经表明，制动可预防犬类前交叉韧带横断后机械诱导性骨关节炎的发生。在此，我们研究腿部制动对豚鼠关节内注射碘乙酸所致化学诱导性股骨关节软骨退变的影响。注射后一周，未制动动物的软骨番红O染色减少，软骨细胞数量减少10%-20%。注射后三周，细胞死亡和番红O染色缺失进一步发展，表面出现纤维化和骨赘。接受碘乙酸注射的豚鼠对侧（未注射）膝关节的关节软骨，以及制动1周但未接受碘乙酸注射的动物膝关节的关节软骨，在组织学和组织化学上均正常。然而，4个未处理且制动3周的膝关节中有2个的标本番红O染色减少。膝关节制动并未改变关节内注射碘乙酸后所见的番红O染色缺失，但确实减少了软骨细胞的耗竭（P<0.05）。此外，在注射碘乙酸后制动的任何动物中均未出现骨赘或纤维化。因此，在这种化学诱导性软骨损伤模型中，制动显然具有保护作用。
